Development of fibronectin-loaded nanofiber scaffolds for guided pulp tissue regeneration

Autor(es)

Leite, Maria Luisa ^[1] ; Soares, Diana Gabriela ^[2] ; Anovazzi, Giovana ^[3] ; Mendes Soares, Igor Paulino ^[1] ; Hebling, Josimeri ^[3] ; de Souza Costa, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil

^[3] Sao Paulo State Univ, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil

^[4] Sao Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, Humaita St 1680, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 109, n. 9, p. 1244-1258, SEP 2021.
• Citações Web of Science: 1

Resumo

Fibronectin (FN)-loaded nanofiber scaffolds were developed and assessed concerning their bioactive potential on human apical papilla cells (hAPCs). First, random (NR) and aligned (NA) nanofiber scaffolds of polycaprolactone (PCL) were obtained by electrospinning technique and their biological properties were evaluated. The best formulations of NR and NA were loaded with 0, 5, or 10 mu g/ml of FN and their bioactivity was assessed. Finally, FN-loaded NR and NA tubular scaffolds were prepared and their chemotactic potential was analyzed using an in vitro model to mimic the pulp regeneration of teeth with incomplete root formation. All scaffolds tested were cytocompatible. However, NR and NA based on 10% PCL promoted the highest hAPCs proliferation, adhesion and spreading. Polygonal and elongated cells were observed on NR and NA, respectively. The higher the concentration of FN added to the scaffolds, greater cell migration, viability, proliferation, adhesion and spreading, as well as collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1). In addition, tubular scaffolds with NA loaded with FN (10 mu g/ml) showed the highest chemotactic potential on hAPCs. It was concluded that FN-loaded NA scaffolds may be an interesting biomaterial to promote hAPCs-mediated pulp regeneration of endodontically compromised teeth with incomplete root formation. (AU)

• Processo FAPESP: 17/22739-9 - Desenvolvimento e análise de um scaffold bicamada para regeneração do complexo dentino-pulpar
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/14210-8 - Síntese e avaliação de biomateriais associados a proteínas da matriz extracelular para a regeneração do tecido pulpar
• Beneficiário: Maria Luísa de Alencar e Silva Leite
• Modalidade de apoio: Bolsas no Brasil - Doutorado