The effects of ionic strength and pH on antibacterial activity of hybrid biosurfactant-biopolymer nanoparticles

Autor(es)

Marangon, Crisiane A. ^[1] ; Vigilato Rodrigues, Murilo A. ^[2] ; Vicente Bertolo, Mirella R. ^[2] ; Amaro Martins, Virginia da C. ^[2] ; de Guzzi Plepis, Ana Maria ^[1] ; Nitschke, Marcia ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Interunits Grad Program Bioengn EESC FMRP IQSC, Trabalhador Sao Carlense Av 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Chem IQSC, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Polymer Science; v. 139, n. 1 JUL 2021.
• Citações Web of Science: 0

Resumo

The physicochemical properties of nanoparticles (NPs) directly influence the colloidal stability and bio-nano interface, which makes the study of these factors extremely important for NPs applicability. Here, we report the influence of pH and ionic strength in the size, surface charge, and antimicrobial activity of chitosan/rhamnolipid nanoparticles (C/RL-NPs), synthesized by mixing C and RL in the proportion of 1:1, with the dropwise addition of TPP (sodium tripolyphosphate) in the molar ratio of 1:2.5:1.3, respectively. The obtained NPs were resuspended in different solvents to investigate the ionic strength effect on their stability and antimicrobial activity. By decreasing pH and ionic strength, NPs size was reduced while their surface charge increased. Chitosan nanoparticles (C-NPs) remained stable in the presence of TPP at acidic pH and low ionic strength, indicating that the modulation of physicochemical conditions for the crosslinking between the two ionic components was an efficient approach to enhance C/RL-NPs colloidal stability. Thermogravimetric analysis confirmed the interaction between rhamnolipid and chitosan in C/RL-NPs. The antibacterial activity of C/RL-NPs against Staphylococcus aureus increased significantly, demonstrating the importance of investigating size and surface charge to dictate the bioactivity properties of such hybrid natural-based nanomaterial. (AU)

• Processo FAPESP: 19/14405-9 - Explorando o potencial de Ramnolipídeos no controle de patógenos alimentares: efeito da força iônica e biopolímeros
• Beneficiário: Marcia Nitschke
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/18748-8 - Composição química de morangos recobertos com biofilmes a base de extratos de casca de romã, quitosana e gelatina
• Beneficiário: Mirella Romanelli Vicente Bertolo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto