Engineering of pH-triggered nanoplatforms based on novel poly(2-methyl-2-oxazoline)-b-poly[2-(diisopropylamino)ethyl methacrylate] diblock copolymers with tunable morphologies for biomedical applications

Autor(es)

Cernoch, Peter ^[1] ; Jager, Alessandro ^[1] ; Cernochova, Zulfiya ^[1] ; Sincari, Vladimir ^[1] ; Albuquerque, Lindomar J. C. ^[2] ; Konefal, Rafal ^[1] ; Pavlova, Ewa ^[1] ; Giacomelli, Fernando C. ^[2] ; Jager, Eliezer ^[1]

Afiliação do(s) autor(es)

^[1] Czech Acad Sci, Inst Macromol Chem, Heyrovsky Sq 2, Prague 16206 - Czech Republic

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Polymer Chemistry; v. 12, n. 19, p. 2868-2880, MAY 21 2021.
• Citações Web of Science: 0

Resumo

A two-step synthetic approach via the combination of living cationic ring-opening (CROP) and reversible addition-fragmentation chain transfer (RAFT) polymerization techniques was used to produce novel amphiphilic block copolymers based on the water-soluble poly(2-methyl-2-oxazoline) (PMeOx), which holds protein repelling properties, linked to the hydrophilic-hydrophobic pH-responsive block poly{[}2-(diisopropylamino)ethyl methacrylate] (PDPA). Hydrodynamic flow focusing nanoprecipitation microfluidics (MF) was further employed to manufacture block copolymer self-assemblies. Interestingly, although all the synthesized macromolecules contained higher amounts of the pH-responsive segment, the microfluidic approach allowed the manufacturing of core-shell micelles and polymersomes. The morphology seems to be driven by the overall molecular weight of the block copolymers rather than by the hydrophilic-to-hydrophobic weight ratio. Longer and shorter amphiphilic chains enabled the manufacturing of core-shell assemblies and polymeric vesicles, respectively. The use of PMeOx and PDPA blocks confers serum stability and pH-responsive behavior to the nanoparticles in a pH window which is particularly useful for tumour detection and therapy. The self-assembled nanostructures are non-toxic even at fairly high polymer concentrations. All these features therefore can be useful in the design of pH-triggered nanoplatforms of distinct morphologies towards a variety of biomedical applications, for instance, the loading and delivery of hydrophobic and hydrophilic therapeutics. (AU)

• Processo FAPESP: 19/06634-8 - Vesículas poliméricas sensíveis a estímulos ambientais como plataformas alternativas em terapias contra o câncer
• Beneficiário: Fernando Carlos Giacomelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/23844-8 - Vesículas poliméricas biodegradáveis e sensíveis ao pH para encapsulação de BIOMACROMOLÉCULAS
• Beneficiário: Lindomar Jose Calumby Albuquerque
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/11261-0 - Síntese dos copolímeros em bloco PHPMA-b-PDPA, PEO-b-PDPA e RGD-PHPMA-b-PDPA via RAFT para produção de vesículas poliméricas sensíveis a pH e posterior encapsulação e entrega de biomacromoléculas hidrofílicas
• Beneficiário: Lindomar Jose Calumby Albuquerque
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado