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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antimicrobially active gelatin/[Mg-Al-CO3 ]-LDH composite films based on clove essential oil for skin wound healing

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Autor(es):
Blanco, Guilherme E. de O. [1] ; de Souza, Clovis W. O. [2] ; Bernardo, Marcela P. [3, 4, 5] ; Zenke, Martin [4, 5] ; Mattoso, Luiz H. C. [3] ; Moreira, Francys K. V. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Fed Univ Sao Carlos UFScar, Dept Mat Engn DEMa, Multifunct Packaging Grp, Sao Carlos, SP - Brazil
[2] Fed Univ Sao Carlos UFScar, Dept Morphol & Pathol DMP, Sao Carlos, SP - Brazil
[3] Embrapa Instrumentat CNPDIA, Natl Nanotechnol Lab Agr LNNA, Sao Carlos, SP - Brazil
[4] Rhein Westfal TH Aachen, Fac Med, Dept Cell Biol, Inst Biomed Engn, Aachen - Germany
[5] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, Aachen - Germany
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: MATERIALS TODAY COMMUNICATIONS; v. 27, JUN 2021.
Citações Web of Science: 0
Resumo

In this study, gelatin, a biocompatible, collagen-derived protein, was combined with clove essential oil (CEO) and hydrotalcite ({[}Mg-Al-CO3]-LDH) nanoplates (D similar to 100nm) as an attempt to develop new antimicrobial wound dressings. CEO-loaded gelatin films covering different hydrotalcite contents (0.5-5.0 wt.%) were obtained by solution casting and characterized by light microscopy, FTIR and UV-vis spectroscopies, tensile and optical tests, water contact angle (WCA) determinations, and disc diffusion assays. CEO formed emulsified morphologies with gelatin over the entire hydrotalcite content range. Hydrotalcite contents larger than 2.5 wt.% were key for retain CEO in the gelatin matrix, which ensured antimicrobial activity for the films against Staphylococcus aureus and Escherichia coli. The UV barrier and mechanical properties of gelatin were significantly increased by CEO and further sustained upon hydrotalcite addition. All the composite films were regarded as hydrophilic materials ( WCA < 75 degrees), which was a preliminary requirement for their in vivo biocompatibility. The films were nontoxic towards HeLa cells, with cell viabilities above 70 %, as determined by MTT assay. These outcomes point out a simple way of using hydrotalcite to produce functional, biocompatible composites based on gelatin and clove essential oil for cutaneous wound healing and other biomedical applications. (AU)

Processo FAPESP: 19/23027-8 - Desenvolvimento e avaliação da interação celular de scaffolds impressos em 3D para reconstrução óssea
Beneficiário:Marcela Piassi Bernardo
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 18/07860-9 - Desenvolvimento de filamentos nanocompósitos poliméricos com propriedades anti-inflamatórias para impressão 3D como substituintes ósseos
Beneficiário:Marcela Piassi Bernardo
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado