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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobially active gelatin/[Mg-Al-CO3 ]-LDH composite films based on clove essential oil for skin wound healing

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Blanco, Guilherme E. de O. [1] ; de Souza, Clovis W. O. [2] ; Bernardo, Marcela P. [3, 4, 5] ; Zenke, Martin [4, 5] ; Mattoso, Luiz H. C. [3] ; Moreira, Francys K. V. [1]
Total Authors: 6
[1] Fed Univ Sao Carlos UFScar, Dept Mat Engn DEMa, Multifunct Packaging Grp, Sao Carlos, SP - Brazil
[2] Fed Univ Sao Carlos UFScar, Dept Morphol & Pathol DMP, Sao Carlos, SP - Brazil
[3] Embrapa Instrumentat CNPDIA, Natl Nanotechnol Lab Agr LNNA, Sao Carlos, SP - Brazil
[4] Rhein Westfal TH Aachen, Fac Med, Dept Cell Biol, Inst Biomed Engn, Aachen - Germany
[5] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, Aachen - Germany
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 0

In this study, gelatin, a biocompatible, collagen-derived protein, was combined with clove essential oil (CEO) and hydrotalcite ({[}Mg-Al-CO3]-LDH) nanoplates (D similar to 100nm) as an attempt to develop new antimicrobial wound dressings. CEO-loaded gelatin films covering different hydrotalcite contents (0.5-5.0 wt.%) were obtained by solution casting and characterized by light microscopy, FTIR and UV-vis spectroscopies, tensile and optical tests, water contact angle (WCA) determinations, and disc diffusion assays. CEO formed emulsified morphologies with gelatin over the entire hydrotalcite content range. Hydrotalcite contents larger than 2.5 wt.% were key for retain CEO in the gelatin matrix, which ensured antimicrobial activity for the films against Staphylococcus aureus and Escherichia coli. The UV barrier and mechanical properties of gelatin were significantly increased by CEO and further sustained upon hydrotalcite addition. All the composite films were regarded as hydrophilic materials ( WCA < 75 degrees), which was a preliminary requirement for their in vivo biocompatibility. The films were nontoxic towards HeLa cells, with cell viabilities above 70 %, as determined by MTT assay. These outcomes point out a simple way of using hydrotalcite to produce functional, biocompatible composites based on gelatin and clove essential oil for cutaneous wound healing and other biomedical applications. (AU)

FAPESP's process: 19/23027-8 - Development of 3D printed scaffolds for bone reconstruction and evaluation of interaction through cellular study
Grantee:Marcela Piassi Bernardo
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 18/07860-9 - Development of nanocomposite polymer filaments with anti-inflammatory properties for 3D printing as bone substituent
Grantee:Marcela Piassi Bernardo
Support type: Scholarships in Brazil - Post-Doctorate