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Evaluation of cellular and molecular mechanisms in skin wound healing using Copaifera langsdorffii hydroalcoholic extract and oil resin ointments

Grant number: 14/23684-5
Support type:Scholarships in Brazil - Master
Effective date (Start): June 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Pharmacy
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Claudia Helena Pellizzon
Grantee:Lucas Fernando Sérgio Gushiken
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated research grant:11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species, AP.TEM

Abstract

This project is part of Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera FAPESP 2011/13630-7 and a continuation of the project Avaliação do processo de cicatrização de lesões cutâneas utilizando extrato hidroalcóolico e óleo de copaíba FAPESP 2012/15092-5. The tegumental system is highly complex, essential to maintaining the integrity of the body, being a protective differentiated barrier. The discontinuity of this system promotes the healing mechanism, physiological process that, after injury, for total epithelial renewal, can be didactically divided into interdependent and overlapping phases: the inflammatory phase, whose main events are hemostasis and inflammation, proliferative phase, in which there is the formation of granulation tissue and phase of remodeling of extracellular matrix. Due to the possibility of some kind of physical disability, psychosocial consequences or even lead to death, the skin lesions have great importance in the investment of public resources. The Copaifera langsdorffii Desf. (Leguminosae) is a native tree of tropical regions of Latin America and West Africa witch, according to popular knowledge, has anti-inflammatory, antimicrobial and wound healing activities. Therefore, this project aims to investigate the effect and mechanisms involved in the healing action of ointments of hydroalcoholic extract of leaves and oil resin of bark of Copaifera langsdorffii in skin wounds of rats. Male Wistar rats will be randomly divided into four experimental groups (n = 8): base lanete ointment (negative control), Collagenase ointment (positive control), hydroalcoholic extract 10% ointment and oil resin 10% ointment. The animals will be anesthetized and then dorsal lesion will be made with a punch of 2 cm of diameter. The lesions will be treated and measured once a day using a digital caliper for 3, 7 and 14 days (according to the phases described in the literature). After euthanasia, the region of the wound will be designed to histological analysis (count of inflammatory cells, inflammatory score, morphology and deposition of collagen fibers), morphometric (height of the epithelium regeneration, thickness of epidermis and dermis) and immunohistochemical (metalloproteinase-2, metalloproteinase-9, EGF and NF-kappa²), determination of collagen, and molecular analyzes (qPCR and RT-PCR) for collagen I and III, and growth factors such as TGF-²1 and EGF. Antioxidant activity will be measured (reduced glutathione, glutathione reductase and peroxidase, superoxide dismutase and myeloperoxidase), anti-inflammatory activity by measuring cytokines in blood serum by ELISA method (TNF-±, IL-1², IL-6 and IL will also be determined -10). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERGIO GUSHIKEN, LUCAS FERNANDO; HUSSNI, CARLOS ALBERTO; BASTOS, JAIRO KENUPP; ROZZA, ARIANE LEITE; BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; PADOVANI, CARLOS ROBERTO; LEMOS, MARIVANE; POLIZELLO JUNIOR, MAURILIO; MANGABEIRA DA SILVA, JONAS JOAQUIM; NOBREGA, RAFAEL HENRIQUE; MONTEIRO MARTINEZ, EMANUEL RICARDO; PELLIZZON, CLAUDIA HELENA. Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats. Evidence-based Complementary and Alternative Medicine, 2017. Web of Science Citations: 2.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GUSHIKEN, Lucas Fernando Sérgio. Avaliação dos mecanismos bioquímicos, celulares e moleculares na cicatrização de lesões cutâneas em ratos utilizando cremes à base de extrato hidroalcoólico e óleo-resina de Copaifera langsdorffii. 2017. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Botucatu)..

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