Grant number: | 22/05953-5 |
Support Opportunities: | Scholarships in Brazil - Doctorate |
Start date until: | October 01, 2022 |
End date until: | September 30, 2026 |
Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery |
Principal Investigator: | Vânia Maria de Vasconcelos Machado |
Grantee: | Talita D'Paula Tavares Pereira Muniz |
Host Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
Abstract Currently, there is an intense search for biocompatible materials that are osteoconductive, osteointegrative, with good biomechanical resistance and low modulus of elasticity to be used safely as orthopedic implants. Therefore, the objective is to evaluate surface modification treatments and effects on the antimicrobial activity of Ti-34Nb-6Sn/Mg ² alloys produced by powder metallurgy for use as biomaterial. Triplicates distributed in eight groups will be analyzed, at three different times (24h, after 3 days and 7 days), characterized by a control group (CG) and 7 different treatment groups between containing only osteogenic medium, evaluation with surface modifications by anodizing with glycerol electrolyte + 0.25% NH4 F at 30V for 9h and annealed at 530ºC (5ºC/min) and immersion coating in 0.3M CaCl2 and 0.5M Na2 HPO4 solutions for hydroxyapatite growth, both on pure titanium as on the surface of the Ti-34Nb-6Sn/Mg alloy. Subsequently, biological characterization will be carried out through adhesion tests, cell viability, extraction of samples for RT-PCR for gene expression, gelatinolytic activity of metalloproteinases, Alizarin Red assay and Wound Healing assay; substrate characterization by scanning electron microscopy (SEM), X-ray diffractometer (XRD) and dynamic contact angle; and electrochemical corrosion tests (ICP-OES). Data normality will be assessed using the Kolmogorov-Smirnov test (p < 0.05). If there is a normal distribution, analysis of variance will be performed to compare the groups within each assessment moment and the One Way Repeated Measures test for each group (p < 0.05). | |
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