Interplay among miR-29 family, mineral metabolism, and gene regulation in Bos indicus muscle

Autor(es)

da Silva Diniz, Wellison Jarles ^{[1, 2]} ; Banerjee, Priyanka ^[1] ; Mazzoni, Gianluca ^[3] ; Coutinho, Luiz Lehmann ^[4] ; Mello Cesar, Aline Silva ^[4] ; Afonso, Juliana ^[2] ; Gromboni, Caio Fernando ^[5] ; Araujo Nogueira, Ana Rita ^[6] ; Kadarmideen, Haja N. ^[1] ; de Almeida Regitano, Luciana Correia ^[6]

Afiliação do(s) autor(es)

^[1] Tech Univ Denmark, Dept Appl Math & Comp Sci, Lyngby - Denmark

^[2] Univ Fed Sao Carlos, Ctr Biol & Hlth Sci CCBS, Grad Program Evolutionary Genet & Mol Biol, Sao Carlos, SP - Brazil

^[3] Tech Univ Denmark, Dept Hlth Technol, Lyngby - Denmark

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Anim Sci, Piracicaba, SP - Brazil

^[5] Bahia Fed Inst Educ Sci & Technol, IFBA, Campus Ilheus, Ilheus, BA - Brazil

^[6] Empresa Brasileira Pesquisa Agr, Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Genetics and Genomics; v. 295, n. 5, p. 1113-1127, SEP 2020.
• Citações Web of Science: 0

Resumo

An interplay between gene expression, mineral concentration, and beef quality traits in Bos indicus muscle has been reported previously under a network approach. However, growing evidence suggested that miRNAs not only modulate gene expression but are also involved with mineral homeostasis. To our knowledge, understanding of the miRNA-gene expression-mineral concentration relationship in mammals is still minimal. Therefore, we carried out a miRNA co-expression and multi-level miRNA-mRNA integration analyses to predict the putative drivers (miRNAs and genes) associated with muscle mineral concentration in Nelore steers. In this study, we identified calcium and iron to be the pivotal minerals associated with miRNAs and gene targets. Furthermore, we identified the miR-29 family (miR-29a, -29b, -29c, -29d-3p, and -29e) as the putative key regulators modulating mineral homeostasis. The miR-29 family targets genes involved with AMPK, insulin, mTOR, and thyroid hormone signaling pathways. Finally, we reported an interplay between miRNAs and minerals acting cooperatively to modulate co-expressed genes and signaling pathways both involved with mineral and energy homeostasis in Nelore muscle. Although we provided some evidence to understand this complex relationship, future work should determine the functional implications of minerals for miRNA levels and their feedback regulation system.\textbackslash{}\textbackslash{}An interplay between gene expression, mineral concentration, and beef quality traits in Bos indicus muscle has been reported previously under a network approach. However, growing evidence suggested that miRNAs not only modulate gene expression but are also involved with mineral homeostasis. To our knowledge, understanding of the miRNA-gene expression-mineral concentration relationship in mammals is still minimal. Therefore, we carried out a miRNA co-expression and multi-level miRNA-mRNA integration analyses to predict the putative drivers (miRNAs and genes) associated with muscle mineral concentration in Nelore steers. In this study, we identified calcium and iron to be the pivotal minerals associated with miRNAs and gene targets. Furthermore, we identified the miR-29 family (miR-29a, -29b, -29c, -29d-3p, and -29e) as the putative key regulators modulating mineral homeostasis. The miR-29 family targets genes involved with AMPK, insulin, mTOR, and thyroid hormone signaling pathways. Finally, we reported an interplay between miRNAs and minerals acting cooperatively to modulate co-expressed genes and signaling pathways both involved with mineral and energy homeostasis in Nelore muscle. Although we provided some evidence to understand this complex relationship, future work should determine the functional implications of minerals for miRNA levels and their feedback regulation system. (AU)

• Processo FAPESP: 17/20761-7 - Análises de integração genética e de redes regulatórias para detectar genes associados com conteúdo de ferro no músculo e fenótipos relacionados a qualidade de carne em Nelore
• Beneficiário: Wellison Jarles da Silva Diniz
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 12/23638-8 - Bases moleculares da qualidade da carne em bovinos da raça Nelore
• Beneficiário: Luciana Correia de Almeida Regitano
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/09158-1 - Identificação de redes gênicas de coexpressão e dos mecanismos regulatórios associados à composição mineral e qualidade de carne em bovinos
• Beneficiário: Wellison Jarles da Silva Diniz
• Modalidade de apoio: Bolsas no Brasil - Doutorado