Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi

Autor(es)

Arruda, Bruna ^{[1, 2]} ; George, Paul B. L. ^{[2, 3]} ; Robin, Agnes ^{[1, 4, 5]} ; de L. C. Mescolotti, Denise ^[1] ; Herrera, Wilfrand F. B. ^[6] ; Jones, Davey L. ^{[2, 7]} ; Andreote, Fernando D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Soil Sci, Luiz de Queiroz Coll Agr, Piracicaba - Brazil

^[2] Bangor Univ, Sch Nat Sci, Bangor LL57 2UW, Gwynedd - Wales

^[3] Univ Laval, Dept Med Mol, Quebec City, PQ - Canada

^[4] CIRAD, UMR Eco&Sols, Piracicaba - Brazil

^[5] Univ Montpellier, Eco&Sols, CIRAD, INRAE, IRD, Montpellier - France

^[6] Colombian Corp Agr Res Agrosavia, Pasto - Colombia

^[7] Univ Western Australia, Sch Agr & Environm, SoilsWest, Perth, WA 6009 - Australia

• Tipo de documento: Artigo Científico
• Fonte: MYCORRHIZA; v. 31, n. 5 AUG 2021.
• Citações Web of Science: 0

Resumo

Arbuscular mycorrhizal fungi (AMF) are important symbionts of many plant species, facilitating the acquisition of soil nutrients by roots. We hypothesized that AMF root colonization is strongly influenced by the composition of the soil microbiome. Here, we evaluated mycorrhizal colonization of two plants, the grass Urochloa brizantha (Brachiaria) and the legume Crotalaria juncea (Crotalaria). These were cultivated in the same soil but hosting eight distinct microbiomes: natural soil (i); soil exposed to heat treatments for 1 h at 50 oC (ii), 80 oC (iii), or 100 oC (iv); sterilized soil by autoclaving (AS) followed by re-inoculation of dilutions of the natural soil community at 10(-1) (v), 10(-3) (vi), and 10(-6) (vii); and AS without re-inoculation (viii). Microbial diversity (bacteria and fungi) was assessed through 16S rDNA and ITS1 metabarcoding, respectively, and the soil acid phosphatase activity (AP(ASE)) was measured. Sequencing results showed the formation of distinct microbial communities according to the soil manipulations, which also correlated with the decline of AP(ASE). Subsequently, seedlings of Brachiaria and Crotalaria were grown in those soils inoculated separately with three AMF (Acaulospora colombiana, Rhizophagus clarus, and Dentiscutata heterogama) which were compared to an AMF-free control treatment. Brachiaria showed higher colonization in natural soil when compared to the microbial community manipulations, regardless of the AMF species inoculated. In contrast, two mycorrhiza species were able to colonize Crotalaria under modified microbial communities at similar rates to natural soil. Furthermore, Brachiaria showed a possible inverse relationship between AP(ASE) and mycorrhization, but this trend was absent for Crotalaria. We conclude that mycorrhizal root colonization and soil acid phosphatase activity were associated with the structure of the soil microbiome, depending on the plant species evaluated. (AU)

• Processo FAPESP: 16/21596-7 - Interação entre a fertilização fosfatada e a diversidade microbiana do solo na colonização de plantas por fungos micorrízicos arbusculares
• Beneficiário: Bruna Arruda
• Modalidade de apoio: Bolsas no Brasil - Doutorado