Optimization of (-)-cubebin biotransformation to (-)-hinokinin by the marine fungus Absidia coerulea 3A9

Autor(es):Mostrar menos -

de Souza, Jonathan Messias ^[1] ; Conceicao Santos, Mario Ferreira ^[1] ; Nascimento Pedroso, Rita Cassia ^[1] ; Pimenta, Leticia Pereira ^[1] ; Siqueira, Katia Aparecida ^[2] ; Soares, Marcos Antonio ^[2] ; Dias, Gustavo Muniz ^[3] ; Linhari Rodrigue Pietro, Rosemeire Cristina ^[4] ; Ramos, Henrique Pereira ^[1] ; Andrade Silva, Marcio Luis ^[1] ; Pauletti, Patricia Mendonca ^[1] ; Sola Veneziani, Rodrigo Cassio ^[1] ; Ambrosio, Sergio Ricardo ^[1] ; Braun, Glaucia Hollaender ^[1] ; Januario, Ana Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Ctr Res Exact & Technol Sci, BR-14404600 Franca, SP - Brazil

^[2] Univ Fed Mato Grosso, Biosci Inst, Dept Bot & Ecol, BR-78060900 Cuiaba, MT - Brazil

^[3] Fed Univ ABC, Ctr Nat & Human Sci, BR-09606070 Sao Bernardo Do Campo, SP - Brazil

^[4] Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Microbiology; v. 203, n. 7, p. 4313-4318, SEP 2021.
• Citações Web of Science: 0

Resumo

The genus Absidia is widely used in the biotransformation of different classes of natural products. This study evaluates the ability of the Absidia coerulea 3A9 marine derived strain isolated from the ascidian Distaplia stilyfera to perform biotransformations by conducting assays with (-)-cubebin, as substrate. The experiment was optimized using the experimental design proposed by Plackett-Burman for seven factors and eight experiments, to establish the biotransformation conditions that would allow maximum production of biotransformed dibenzylbutyrolactone (-)-hinokinin. An analytical method based on Reverse-Phase-High Performance Liquid Chromatography (RP-HPLC) was developed to quantify the fungal biotransformation product. The factor that influenced the (-)-hinokinin peak area the most positively was the percentage of seawater (%seawater) given that its %relative standard deviation (%RSD) showed a 32.92% deviation from the real value. (AU)

• Processo FAPESP: 14/19184-7 - Investigação do potencial esquistossomicida de invertebrados marinhos pertencentes à Ascidiacea
• Beneficiário: Ana Helena Januário
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/14261-1 - Investigação do potencial esquistossomicida, leishmanicida e citototóxico de linhagens fúngicas associadas a invertebrados marinhos pertencentes a Ascidiacea
• Beneficiário: Ana Helena Januário
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/54762-8 - Obtenção de análogos estruturais do ácido pimaradienóico através do processo de biotransformação fúngica e estudo do efeito destes metabólitos sobre a atividade da enzima TcDHODH e sobre a contração da musculatura lisa vascular de artéria aorta de ratos
• Beneficiário: Sergio Ricardo Ambrosio
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores