Optimization of (-)-cubebin biotransformation to (-)-hinokinin by the marine fungus Absidia coerulea 3A9

Author(s):Show less -

de Souza, Jonathan Messias ^[1] ; Conceicao Santos, Mario Ferreira ^[1] ; Nascimento Pedroso, Rita Cassia ^[1] ; Pimenta, Leticia Pereira ^[1] ; Siqueira, Katia Aparecida ^[2] ; Soares, Marcos Antonio ^[2] ; Dias, Gustavo Muniz ^[3] ; Linhari Rodrigue Pietro, Rosemeire Cristina ^[4] ; Ramos, Henrique Pereira ^[1] ; Andrade Silva, Marcio Luis ^[1] ; Pauletti, Patricia Mendonca ^[1] ; Sola Veneziani, Rodrigo Cassio ^[1] ; Ambrosio, Sergio Ricardo ^[1] ; Braun, Glaucia Hollaender ^[1] ; Januario, Ana Helena ^[1]

Affiliation

^[1] Univ Franca, Ctr Res Exact & Technol Sci, BR-14404600 Franca, SP - Brazil

^[2] Univ Fed Mato Grosso, Biosci Inst, Dept Bot & Ecol, BR-78060900 Cuiaba, MT - Brazil

^[3] Fed Univ ABC, Ctr Nat & Human Sci, BR-09606070 Sao Bernardo Do Campo, SP - Brazil

^[4] Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, BR-14800903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 203, n. 7, p. 4313-4318, SEP 2021.
• Web of Science Citations: 0

Abstract

The genus Absidia is widely used in the biotransformation of different classes of natural products. This study evaluates the ability of the Absidia coerulea 3A9 marine derived strain isolated from the ascidian Distaplia stilyfera to perform biotransformations by conducting assays with (-)-cubebin, as substrate. The experiment was optimized using the experimental design proposed by Plackett-Burman for seven factors and eight experiments, to establish the biotransformation conditions that would allow maximum production of biotransformed dibenzylbutyrolactone (-)-hinokinin. An analytical method based on Reverse-Phase-High Performance Liquid Chromatography (RP-HPLC) was developed to quantify the fungal biotransformation product. The factor that influenced the (-)-hinokinin peak area the most positively was the percentage of seawater (%seawater) given that its %relative standard deviation (%RSD) showed a 32.92% deviation from the real value. (AU)

• FAPESP's process: 14/19184-7 - Schistosomicidal potential investigation of marine invertebrates belonging to the Ascidiacea family
• Grantee: Ana Helena Januário
• Support type: Regular Research Grants
• FAPESP's process: 17/14261-1 - Investigation of the schistosomicidal, leishmanicidal and cytototoxic potential of fungal lineages associated with marine invertebrates belonging to Ascidiacea
• Grantee: Ana Helena Januário
• Support type: Regular Research Grants
• FAPESP's process: 07/54762-8 - Obtaining structural analogues of pimaradienoic acid through the process of fungal biotransformation and study of the effect of these metabolites on the activity of the TcDHODH enzyme and on the contraction of the vascular smooth muscle of the aorta artery of mice
• Grantee: Sergio Ricardo Ambrosio
• Support type: BIOTA-FAPESP Program - Young Investigators Grants