In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins

Autor(es)

Srimahaeak, Thanyaporn ^[1] ; Bianchi, Fernanda ^{[1, 2]} ; Chlumsky, Ondrej ^[3] ; Larsen, Nadja ^[1] ; Jespersen, Lene ^[1]

Afiliação do(s) autor(es)

^[1] Univ Copenhagen, Fac Sci, Dept Food Sci, Frederiksberg - Denmark

^[2] UNESP Sao Paulo State Univ, Dept Food Sci, Araraquara, SP - Brazil

^[3] Masaryk Univ, Dept Expt Biol, Brno - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: Journal of Functional Foods; v. 79, APR 2021.
• Citações Web of Science: 0

Resumo

This study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54?73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 ? 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier. (AU)

• Processo FAPESP: 16/20336-1 - Efeito da atividade simbiótica sob a integridade do epitélio intestinal em microbiota obesa e eutrófica
• Beneficiário: Fernanda Bianchi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado