In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins

Author(s)

Srimahaeak, Thanyaporn ^[1] ; Bianchi, Fernanda ^{[1, 2]} ; Chlumsky, Ondrej ^[3] ; Larsen, Nadja ^[1] ; Jespersen, Lene ^[1]

Affiliation

^[1] Univ Copenhagen, Fac Sci, Dept Food Sci, Frederiksberg - Denmark

^[2] UNESP Sao Paulo State Univ, Dept Food Sci, Araraquara, SP - Brazil

^[3] Masaryk Univ, Dept Expt Biol, Brno - Czech Republic

• Document type: Journal article
• Source: Journal of Functional Foods; v. 79, APR 2021.
• Web of Science Citations: 0

Abstract

This study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54?73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 ? 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier. (AU)

• FAPESP's process: 16/20336-1 - Effect of synbiotic activity on intestinal epithelial integrity in obese and lean microbiota
• Grantee: Fernanda Bianchi
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate