Oral shedding of CMV and HSV-1 in hematopoietic stem cell transplantation patients

Autor(es):Mostrar menos -

Costa, Andre L. F. ^[1] ; Santos, Bruna A. ^[2] ; Torregrossa, Vinicius R. ^[2] ; Miranda, Eliana C. M. ^[2] ; Vigorito, Afonso C. ^[2] ; Palmieri, Michelle ^[3] ; Ricardo, Ana L. F. ^[1] ; Sarmento, Dmitry J. S. ^{[3, 4]} ; Mamana, Ana C. ^[5] ; Tozetto-Mendoza, Tania R. ^[5] ; Correa, M. Elvira P. ^[2] ; Braz-Silva, Paulo H. ^{[3, 5]}

Afiliação do(s) autor(es)

^[1] Cruzeiro Do Sul Univ UNICSUL, Postgrad Program Dent, Sao Paulo - Brazil

^[2] Univ Campinas UNICAMP, Hematol & Hemotherapy Ctr, Rua Carlos Chagas 480, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil

^[4] State Univ Paraiba, Sch Dent, Dept Stomatol, Araruna - Brazil

^[5] Univ Sao Paulo, Sch Med, Inst Trop Med Sao Paulo, Lab Virol LIM52, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 27, n. 6, p. 1572-1579, SEP 2021.
• Citações Web of Science: 0

Resumo

Objectives To evaluate the oral shedding of herpesviruses in patients undergoing hematopoietic stem cell transplantation (HSCT) and correlate it with oral mucositis (OM). Methods Saliva samples were collected before the HSCT and on day D + 8. Multiplex Polymerse Chain Reaction (PCR) was performed to detect herpes simplex virus (HSV)-1 and HSV-2, Epstein-Barr virus (EBV), Cytomegalovirus (CMV), Variella-zoster virus (VZV), and human herpesvirus (HHV)-6, HHV-7, and HHV-8. OM was assessed according to WHO criteria. Results Thirty one patients were enrolled, in which 20 of 31 (64.5%) were males; median age was 50 (21-70) years; 16 of 31 (51.6%) underwent allo-HSCT; and 15 of 31 (48.4%) underwent auto-HSCT. On D + 8, OM grades III and IV were observed in 8 of 31 (25.8%) patients. In the first salivary collection, EBV was found in 24 of 31 (77.4%), followed by HHV-6 (7/31, 22.6%) and HHV-7 (8/31 25.8%). In the second collection, EBV was found in 24 of 27(89%), followed by HSV-1 (8/27, 30%) and CMV, HHV-6, and HHV-7 (5/27, 18.5%, each one). On D + 8, OM grades II and IV were associated with the presence of HSV-1. HSV-1 was also associated with worsening degrees of OM on D + 15. Conclusion The presence of HSV-1 and CMV in oral samples was more frequent on day D + 8 after HSCT. HSV-1 detection was associated with severity and worsening of OM. HSV-1 and CMV seem to be associated with oral dysbiosis due to HSCT. (AU)

• Processo FAPESP: 15/07727-9 - Avaliação do ambiente imunoregulatório de lesões potencialmente malignas de boca, carcinomas de boca e orofaringe relacionados ou não ao HPV.
• Beneficiário: Paulo Henrique Braz da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular