In vivo effectiveness of hybrid membranes with osteogenic growth peptide for bone regeneration

Autor(es):Mostrar menos -

Oshiro-Junior, Joao Augusto ^{[1, 2]} ; Barros, Rafaela Moreno ^[1] ; da Silva, Camila Garcia ^[2] ; de Souza, Caroline Cordeiro ^[3] ; Scardueli, Cassio Rocha ^{[1, 2]} ; Marcantonio, Camila Chierici ^[3] ; da Silva Saches, Paulo Ricardo ^[4] ; Mendes, Larissa ^[4] ; Cilli, Eduardo Maffud ^[4] ; Chierici Marcantonio, Rosemary Adriana ^[3] ; Chiavacci, Leila Aparecida ^[2]

Afiliação do(s) autor(es)

^[1] State Univ Paraiba, Grad Program Pharmaceut Sci, Campina Grande, Paraiba - Brazil

^[2] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Interstate Highway Araraquara Jau, Km 01, S-N, Campo, BR-14800903 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Fac Dent, Araraquara, SP - Brazil

^[4] Sao Paulo State Univ, UNESP, Chem Inst, Dept Biochem & Chem Technol, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE; v. 15, n. 8, p. 722-731, AUG 2021.
• Citações Web of Science: 0

Resumo

Guided bone regeneration (GBR) technique helps to restore bone tissue through cellular selectivity principle. Currently no osteoinductive membrane exists on the market. Osteogenic growth peptide (OGP) acts as a hematopoietic stimulator. This association could improve the quality of bone formation, benefiting more than 2.2 million patients annually. The objective of this work was to develop membranes from ureasil-polyether materials containing OGP. The membranes were characterized by differential scanning calorimetry (DSC) and small angle X-ray scattering (SAXS). OGP was synthesized by the solid phase method. Sterilization results using gamma radiation at 24 kGy did not change the structure of the material, as confirmed by DSC. The SAXS technique revealed the structural homogeneity of the matrix. OGP was incorporated in 66.25 x 10(-10) mol and release results showed that the ureasil-PPO400/PEO500 and ureasil-PPO400/PEO1900 membranes released 7% and 21%, respectively, after 48 h. In vivo results demonstrated that the amount and quality of bone tissue formed in the bone defects in the presence of ureasil-polyether membranes with OGP were similar to commercial collagen material with BMP. The results allow us to conclude that membranes with OGP have characteristics that make them potential candidates for the GBR. (AU)

• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs