Rondonin: antimicrobial properties and mechanism of action

Autor(es)

Riciluca, Katie C. T. ^{[1, 2]} ; Oliveira, Ursula C. ^[1] ; Mendonca, Ronaldo Z. ^[3] ; Bozelli Junior, Jose C. ^{[4, 5]} ; Schreier, Shirley ^[4] ; da Silva Junior, Pedro I. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Butantan Inst, Lab Appl Toxinol, Ctr Toxins Immune Response & Cell Signaling CeTIC, BR-05503900 Sao Paulo, SP - Brazil

^[2] Post Grad Program Interunits Biotechnol USP IPT I, Sao Paulo - Brazil

^[3] Butantan Inst, Parasitol Lab, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[5] McMaster Univ, Hlth Sci Ctr, Dept Biochem & Biomed Sci, Hamilton, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: FEBS OPEN BIO; v. 11, n. 9, SI AUG 2021.
• Citações Web of Science: 0

Resumo

Infectious diseases are among the major causes of death in the human population. A wide variety of organisms produce antimicrobial peptides (AMPs) as part of their first line of defense. A peptide from Acanthoscurria rondoniae plasma, rondonin-with antifungal activity, a molecular mass of 1236 Da and primary sequence IIIQYEGHKH-was previously studied (UniProt accession number B3EWP8). It showed identity with the C terminus of subunit `D' of the hemocyanin of the Aphonopelma hentzi spider. This result led us to propose a new pathway of the immune system of arachnids that suggests a new function to hemocyanin: production of antimicrobial peptides. Rondonin does not interact with model membranes and was able to bind to yeast nucleic acids but not bacteria. It was not cytotoxic against mammalian cells. The antifungal activity of rondonin is pH-dependent and peaks at pH similar to 4-5. The peptide presents synergism with gomesin (spider hemocyte antimicrobial peptide-UniProtKB-P82358) against human yeast pathogens, suggesting a new potential alternative treatment option. Antiviral activity was detected against RNA viruses, measles, H1N1, and encephalomyocarditis. This is the first report of an arthropod hemocyanin fragment with activity against human viruses. Currently, it is vital to invest in the search for natural and synthetic antimicrobial compounds that, above all, present alternative mechanisms of action to first-choice antimicrobials. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs