| Texto completo | |
| Autor(es): |
Bagatelli, Felipe F. M.
[1]
;
de Luna Vitorino, Francisca N.
[2, 3]
;
da Cunha, Julia P. C.
[2, 3]
;
Oliveira, Carla C.
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP - Brazil
[2] Butantan Inst, Ctr Toxins Immune Response & Cell Signaling, BR-05503900 Sao Paulo, SP - Brazil
[3] Butantan Inst, Lab Cell Cycle, BR-05503900 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Nucleic Acids Research; v. 49, n. 12, p. 7053-7074, JUL 9 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Eukaryotic ribosome biogenesis is an elaborate process during which ribosomal proteins assemble with the pre-rRNA while it is being processed and folded. Hundreds of assembly factors (AF) are required and transiently recruited to assist the sequential remodeling events. One of the most intricate ones is the stepwise removal of the internal transcribed spacer 2 (ITS2), between the 5.8S and 25S rRNAs, that constitutes together with five AFs the pre-60S `foot'. In the transition from nucleolus to nucleoplasm, Nop53 replaces Erb1 at the basis of the foot and recruits the RNA exosome for the ITS2 cleavage and foot disassembly. Here we comprehensively analyze the impact of Nop53 recruitment on the pre-60S compositional changes. We show that depletion of Nop53, different from nop53 mutants lacking the exosome-interacting motif, not only causes retention of the unprocessed foot in late pre-60S intermediates but also affects the transition from nucleolar state E particle to sub-sequent nuclear stages. Additionally, we reveal that Nop53 depletion causes the impairment of late maturation events such as Yvh1 recruitment. In light of recently described pre-60S cryo-EM structures, our results provide biochemical evidence for the structural role of Nop53 rearranging and stabilizing the foot interface to assist the Nog2 particle formation. (AU) | |
| Processo FAPESP: | 15/05776-2 - Caracterização funcional de Nop53/PICT1: modulação da biogênese ribossomal, atividade do exossomo, e efeito sobre o ciclo celular |
| Beneficiário: | Felipe Franco de Melo Bagatelli |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 15/06477-9 - Estudo funcional de componentes do exossomo e do spliceossomo de Saccharomyces cerevisiae e de seu papel no controle pós-transcricional de expressão gênica |
| Beneficiário: | Carla Columbano de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular |
| Beneficiário: | Hugo Aguirre Armelin |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |