mall extracellular vesicles derived from in vivo- or in vitro-produced bovine blastocysts have different miRNAs profiles-Implications for embryo-maternal recognitio

Autor(es):Mostrar menos -

Bridi, Alessandra ^[1] ; Andrade, Gabriella M. ^[1] ; del Collado, Maite ^[1] ; Sangalli, Juliano R. ^[1] ; de Avila, Ana C. F. C. M. ^[1] ; Motta, Igor G. ^[2] ; da Silva, Julio C. B. ^[1] ; Pugliesi, Guilherme ^[2] ; Silva, Luciano A. ^[1] ; Meirelles, Flavio V. ^[1] ; da Silveira, Juliano C. ^[1] ; Perecin, Felipe ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Reproduction and Development; v. 88, n. 9 AUG 2021.
• Citações Web of Science: 1

Resumo

In vivo- and in vitro-produced bovine embryos have different metabolic profiles and differences in gene transcription patterns. These embryos also have a distinct ability to establish and sustain early pregnancies. Small extracellular vesicles (sEVs) are secreted by embryos and carry bioactive molecules, such as miRNAs. We hypothesize that in vivo or in vitro-produced bovine hatched blastocysts on Day 9 and the sEVs secreted by them have different miRNA profiles. To address this hypothesis, embryos of both groups were placed in in vitro culture on Day 7. After 48 h, hatched embryos and hatched embryo-conditioned media (eCM) of both groups were collected. A total of 210 miRNAs were detected in embryos of both groups, of these 6 miRNAs were downregulated, while 7 miRNAs were upregulated in vitro group when compared to in vivo group. sEVs were isolated from eCM to determine miRNA profile. A total of 106 miRNAs were detected in both groups, including 14 miRNAs upregulated in sEVs from in vivo-eCM, and 2 miRNAs upregulated in sEVs from in vitro-eCM. These miRNAs express in embryos and sEVs secreted by them regulate early embryonic developmental and endometrial pathways, which can modify embryo-maternal communication during early pregnancy and consequently affect pregnancy establishment. (AU)

• Processo FAPESP: 18/13155-6 - Mecanismos de acúmulo lipídico oocitário em ambientes metabolicamente alterados: maturação in vitro e obesidade
• Beneficiário: Felipe Perecin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/21829-9 - EMU concedido no projeto 2014/22887-0: NanoSight LM10
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 17/19681-9 - Vesículas extracelulares secretadas por embriões bovinos produzidos in vivo e in vitro: conteúdo de miRNAs e os efeitos moleculares sobre o endométrio e o corpo lúteo
• Beneficiário: Alessandra Bridi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores