Homeobox gene amplification and methylation in oral squamous cell carcinoma

Autor(es)

Rodrigues, Maria Fernanda Setubal Destro ^[1] ; Xavier, Flavia Calo Aquino ^[2] ; Esteves, Carina Duarte ^[3] ; Nascimento, Rebeca Barros ^[2] ; Nobile, Juliana Stephan ^[1] ; Severino, Patricia ^[4] ; de Cicco, Rafael ^[5] ; Toporcov, Tatiana Natasha ^[6] ; Tajara, Eloiza Helena ^[7] ; Nunes, Fabio Daumas ^[3]

Afiliação do(s) autor(es)

^[1] Nove de Julho Univ UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

^[2] Univ Fed Bahia, Sch Dent, Lab Oral Surg Pathol, Salvador, BA - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Sao Paulo - Brazil

^[4] Hosp Israelita Albert Einstein, Albert Einstein Res & Educ Inst, Ctr Expt Res, Sao Paulo - Brazil

^[5] Arnaldo Vieira Carvalho Canc Inst, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, Sao Paulo - Brazil

^[7] Sch Med Sao Jose Rio Preto, Dept Mol Biol, Sch Med, FAMERP, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 129, SEP 2021.
• Citações Web of Science: 0

Resumo

Objectives: Investigate the DNA copy number and the methylation profile of the homeobox genes HOXA5, HOXA7, HOXA9, HOXB5, HOXB13, HOXC12, HOXC13, HOXD10, HOXD11, IRX4 and ZHX1, and correlate them with clinicopathological parameters and overall survival. Material and methods: DNA from OSCC samples and surgical margins were submitted to DNA amplification by qPCR and to DNA methylation analysis using a DNA Methylation PCR Array System. Results: HOXA5, HOXB5 and HOXD10 were amplified in surgical margins while HOXA9, HOXB13 and IRX4 were amplified in OSCC. HOXD10 demonstrated hypermethylation in half of the tumor while ZHX1 did not show hypermethylation. No correlation of DNA copy number or methylation with clinicopathological parameters or survival was observed. Conclusion: HOXA9, HOXB13 and IRX4 genes appears to be regulated by amplification and HOXD10 by methylation in OSCC. Further studies are needed to determine the role of these events in OSCC development. (AU)

• Processo FAPESP: 13/23707-2 - Expressão dos marcadores de células-tronco CD44, OCT4 e BMI-1 em carcinomas epidermóides de boca
• Beneficiário: Camilla Antunes Lopes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica