IL6 and FAS/FASL gene polymorphisms may be associated with disease progression in HIV-1-positive ethnically mixed patients

Autor(es)

Loureiro dos Reis, Marilia M. ^[1] ; Queiroz, Maria A. F. ^[2] ; da Silva, Bosco C. M. ^[3] ; da Silva Duarte, Alberto J. ^[3] ; Arganaraz, Gustavo A. ^[1] ; Vallinoto, Antonio C. R. ^[2] ; Arganaraz, Enrique R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Brasilia, Fac Hlth Sci, Lab Mol Neurovirol, BR-70910900 Brasilia, DF - Brazil

^[2] Fed Univ Para, Inst Biol Sci, Virus Lab, Belem, Para - Brazil

^[3] Univ Sao Paulo, Fac Med FMUSP, Med Invest Lab Unit 56 LIM 56, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Virology; v. 92, n. 8, p. 1148-1157, AUG 2020.
• Citações Web of Science: 2

Resumo

The progression of AIDS depends on the complex host and virus interactions. The most important disease progression hallmarks are immune activation and apoptosis. In this study, we address the prevalence of polymorphisms related to proinflammatory and apoptotic genes, such as IFNG (+874T/A), TNF (308G/A), IL6 (-174G/C), IL8 (-251A/T), FAS (-670A/G), and FASL (-124A/G) in 160 ethnically mixed HIV-1-infected patients from multicentre cohorts with different clinical outcomes (13 elite controllers {[}EC], 66 slow long-term non-progressors {[}LTNPs], and 81 progressors {[}P]). The genotyping was accomplished by TaqMan-qPCR. Among all the polymorphisms analyzed in the cytokines, the IL6 -174G/C polymorphism showed a higher frequency of GG genotype in the LTNP and LTNP+EC groups as compared to the P group. Moreover, there was a significantly higher frequency of the G allele in the LTNP and LTNP+EC groups as compared to the P group. On the other hand, the levels of CD4(+) T lymphocytes were higher among individuals showing the AA and AG genotypes for the FASL -124A/G polymorphism as compared to the GG genotype. Furthermore, the AG and AA genotypes were more frequent, as compared to the GG genotype, in individuals showing a lower viral load. In contrast, for the FAS -670A/G polymorphism, a significantly higher viral load was observed in individuals with the AG genotype as compared to the GG genotype. In conclusion, we found three genetic allelic variants of the IL6 -174G/C, FASL -124A/G, and FAS -670A/G polymorphisms that were related to disease progression and immunological and virological markers in cohorts of HIV-1-positive ethnically mixed patients. (AU)

• Processo FAPESP: 14/09623-3 - Avaliação da resposta imune celular HIV-1 específica, identificação de epitopos do HIV-1 e papel das Células Treg (CD4+CD25+FOXP3+) em indivíduos infectados não progressores por longo tempo (LTNP) e controladores de elite (EC)
• Beneficiário: Bosco Christiano Maciel da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado