DNA Damage, n-3 Long-Chain PUFA Levels and Proteomic Profile in Brazilian Children and Adolescents

Autor(es):Mostrar menos -

de Barros, Tamiris Trevisan ^[1] ; Venancio, Vinicius de Paula ^[2] ; Hernandes, Livia Cristina ^[2] ; Greggi Antunes, Lusania Maria ^[2] ; Hillesheim, Elaine ^[1] ; Salomao, Roberta Garcia ^[1] ; Mathias, Mariana Giaretta ^[1] ; Coelho-Landell, Carolina Almeida ^[1] ; Donega Toffano, Roseli Borges ^[1] ; Ribeiro do Vale Almada, Maria Olimpia ^[1] ; Camelo-Junior, Jose Simon ^[1] ; Moco, Sofia ^[3] ; Cominetti, Ornella ^[3] ; Ued, Fabio da Veiga ^[4] ; Kaput, Jim ^[3] ; Monteiro, Jacqueline Pontes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Pediat, BR-14049900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14049900 Sao Paulo - Brazil

^[3] Soc Produits Nestle SA, Nestle Res, EPFL Innovat Pk, CH-1015 Lausanne - Switzerland

^[4] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Hlth Sci, BR-14049900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 13, n. 8 AUG 2021.
• Citações Web of Science: 0

Resumo

Fatty acids play a significant role in maintaining cellular and DNA protection and we previously found an inverse relationship between blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DNA damage. The aim of this study was to explore differences in proteomic profiles, for 117 pro-inflammatory proteins, in two previously defined groups of individuals with different DNA damage and EPA and DHA levels. Healthy children and adolescents (n = 140) aged 9 to 13 years old in an urban area of Brazil were divided by k-means cluster test into two clusters of DNA damage (tail intensity) using the comet assay (cluster 1 = 5.9% +/- 1.2 and cluster 2 = 13.8% +/- 3.1) in our previous study. The cluster with higher DNA damage and lower levels of DHA (6.2 +/- 1.6 mg/dL; 5.4 +/- 1.3 mg/dL, p = 0.003) and EPA (0.6 +/- 0.2 mg/dL; 0.5 +/- 0.1 mg/dL, p < 0.001) presented increased expression of the proteins CDK8-CCNC, PIK3CA-PIK3R1, KYNU, and PRKCB, which are involved in pro-inflammatory pathways. Our findings support the hypothesis that low levels of n-3 long-chain PUFA may have a less protective role against DNA damage through expression of pro-inflammatory proteins, such as CDK8-CCNC, PIK3CA-PIK3R1, KYNU, and PRKCB. (AU)

• Processo FAPESP: 12/20421-8 - Nova estratégia para analisar interação gene-nutriente em crianças e adolescentes
• Beneficiário: Jacqueline Pontes Monteiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular