Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil

Autor(es)

Martins, Thais P. ^{[1, 2]} ; Souza, Tadeu A. ^[1] ; da Silva, Patricia S. ^[1] ; Nakasu, Erich Y. T. ^[1] ; Melo, Fernando L. ^[2] ; Inoue-Nagata, Alice K. ^[1] ; Nagata, Tatsuya ^[2]

Afiliação do(s) autor(es)

^[1] Embrapa Vegetables, Lab Virol, Brasilia, DF - Brazil

^[2] Univ Brasilia, Dept Mol Biol, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 166, n. 11 SEP 2021.
• Citações Web of Science: 0

Resumo

During a survey in a tomato field in Luziania (Goias State, Brazil), a single plant with mottling, chlorotic spots, and leaf distortion was found. A new bipartite begomovirus sequence was identified using nanopore sequence technology and confirmed by Sanger sequencing. The highest nucleotide sequence identity match of the DNA-A component (2596 bases) was 81.64% with tomato golden leaf deformation virus (HM357456). Due to the current species demarcation criterion of 91% nucleotide sequence identity for DNA-A, we propose this virus to be a new member of the genus Begomovirus, named ``tomato mottle leaf distortion virus{''}. (AU)

• Processo FAPESP: 18/18274-3 - Begomovirus e crinivirus em solanáceas: epidemiologia molecular regional e alternativas sustentáveis de manejo integrado
• Beneficiário: Jorge Alberto Marques Rezende
• Modalidade de apoio: Auxílio à Pesquisa - Temático