Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats

Autor(es)

Luna, G. L. Flores ^[1] ; Oehlmeyer, T. L. ^[1] ; Brandao, G. ^[1] ; Brassolatti, P. ^[2] ; Tosta, J. ^[1] ; Goto, L. S. ^[3] ; de Avo, L. ^[4] ; Leal, A. M. de Oliveira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Med, Lab Invest Endocrina Metab, Postgrad Program Biotechnol, Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Morfol & Patol, Lab Inflamacao & Doencas Infecciosas, Sao Carlos, SP - Brazil

^[3] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias, Campinas, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Med, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 54, n. 11 2021.
• Citações Web of Science: 0

Resumo

Diabetes mellitus is associated with neural and micro- and macrovascular complications. Therapeutic options for these complications are limited and the delivery of mesenchymal stem cells into lesions have been reported to improve the healing process. In this work, the effects of the administration of a lineage of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase (hBMSC-TERT) as a potential therapeutic tool for wound healing in diabetic rats were investigated. This is the first description of the use of these cells in diabetic wounds. Dorsal cutaneous lesions were made in streptozotocin-induced diabetic rats and hBMSC-TERT were subcutaneously administered around the lesions. The healing process was evaluated macroscopically, histologically, and by birefringence analysis. Diabetic wounded rats infused with hBMSC-TERT (DM-TERT group) and the non-diabetic wounded rats not infused with hBMSC-TERT (CW group) had very similar patterns of fibroblastic response and collagen proliferation indicating improvement of wound healing. The result obtained by birefringence analysis was in accordance with that obtained by the histological analysis. The results indicated that local administration of hBMSC-TERT in diabetic wounds improved the wound healing process and may become a therapeutic option for wounds in individuals with diabetes. (AU)

• Processo FAPESP: 16/07423-2 - Tratamento de ferida com hBMSC-TERT em modelo experimental de Diabetes Mellitus
• Beneficiário: Giovanni Pavanello Brandão
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica