Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats

Full text
Author(s):
Luna, G. L. Flores [1] ; Oehlmeyer, T. L. [1] ; Brandao, G. [1] ; Brassolatti, P. [2] ; Tosta, J. [1] ; Goto, L. S. [3] ; de Avo, L. [4] ; Leal, A. M. de Oliveira [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos, Dept Med, Lab Invest Endocrina Metab, Postgrad Program Biotechnol, Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Morfol & Patol, Lab Inflamacao & Doencas Infecciosas, Sao Carlos, SP - Brazil
[3] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias, Campinas, SP - Brazil
[4] Univ Fed Sao Carlos, Dept Med, Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 54, n. 11 2021.
Web of Science Citations: 0
Abstract

Diabetes mellitus is associated with neural and micro- and macrovascular complications. Therapeutic options for these complications are limited and the delivery of mesenchymal stem cells into lesions have been reported to improve the healing process. In this work, the effects of the administration of a lineage of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase (hBMSC-TERT) as a potential therapeutic tool for wound healing in diabetic rats were investigated. This is the first description of the use of these cells in diabetic wounds. Dorsal cutaneous lesions were made in streptozotocin-induced diabetic rats and hBMSC-TERT were subcutaneously administered around the lesions. The healing process was evaluated macroscopically, histologically, and by birefringence analysis. Diabetic wounded rats infused with hBMSC-TERT (DM-TERT group) and the non-diabetic wounded rats not infused with hBMSC-TERT (CW group) had very similar patterns of fibroblastic response and collagen proliferation indicating improvement of wound healing. The result obtained by birefringence analysis was in accordance with that obtained by the histological analysis. The results indicated that local administration of hBMSC-TERT in diabetic wounds improved the wound healing process and may become a therapeutic option for wounds in individuals with diabetes. (AU)

FAPESP's process: 16/07423-2 - Wound healing therapy with hBMSC-TERT in experimental model of Diabetes Mellitus
Grantee:Giovanni Pavanello Brandão
Support Opportunities: Scholarships in Brazil - Scientific Initiation