| Texto completo | |
| Autor(es): |
da Silva, Darlan Barbosa
[1]
;
da Silva, Carolina Loureiro
[1]
;
Davanzo, Nathalia Nossi
[2]
;
Souza, Rodrigo da Silva
[3]
;
Correa, Rodrigo Jose
[3]
;
Tedesco, Antonio Claudio
[2]
;
Riemma Pierre, Maria Bernadete
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Fed Rio de Janeiro, Sch Pharm, Av Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ - Brazil
[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Photobiol & Photomed Res Grp, Av Bandeirantes 3900, BR-14040901 Vila Monte Alegre - Brazil
[3] Univ Fed Rio de Janeiro, Inst Quim, Rio De Janeiro - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Introduction: Nanoparticles (Np) can increase drug efficacy and overcome problems associated with solubility and aggregation in a solution of PpIX. Purpose: Evaluate if Np interferes in the photophysical and photobiological capacity of the PpIX comparing with free PpIX intended for topical PDT of melanoma. Methods: In vitro photophysical evaluation of Np-PpIX was carried out through singlet oxygen (O-1(2)) quantum yield. In vitro cytotoxicity and phototoxicity assays have used murine melanoma cell culture. Results: The quantum yield of singlet oxygen has shown that Np did not influence the formation capacity of this reactive species. In the dark, all PpIX-Nps concentrations were less cytotoxic compared to free drugs. At a higher light dose (1500 mJ.cm(2)) 3.91 mu g/mL PpIX had similar % viable cells for free and Np (similar to 34 %) meaning Nps did not interfere in the photodynamic effect of PpIX. However, at 7.91 mu g/mL the phototoxicity increased for both (5.8 % viable cells for free versus 21.7 % for Nps). Despite the higher phototoxicity of free PpIX at this concentration, greater cytotoxicity in the dark was obtained (similar to 49 % viable cells for free versus similar to 90 .6 % Np) which means Nps protect the tumor tissue from the photodynamic action of PpIX. Conclusions: Np is a potential delivery system for melanoma skin cancer, since it maintained the photophysical properties of PpIX and excellent in vitro phototoxicity effect against melanoma cells, reducing cell viability similar to 80 % (7.91 mu g/mL PpIX in Nps) and provides safe PDT (due to lower cytotoxicity in the dark). (AU) | |
| Processo FAPESP: | 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central |
| Beneficiário: | Antonio Claudio Tedesco |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 15/16660-5 - Estudo de nanoemulsões associadas a terapia fotodinâmica no tratamento do câncer de mama |
| Beneficiário: | Marilia de Freitas Calmon |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |