ass spectrometry-based metabolomics in microbiome investigation

Autor(es)

Bauermeister, Anelize ^{[1, 2]} ; Mannochio-Russo, Helena ^{[3, 2]} ; Costa-Lotufo, Leticia V. ^[1] ; Jarmusch, Alan K. ^[2] ; Dorrestein, Pieter C. ^{[2, 4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[2] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, Collaborat Mass Spectrometry Innovat Ctr, San Diego, CA 92103 - USA

^[3] Sao Paulo State Univ, Inst Chem, Dept Biochem & Organ Chem, Araraquara, SP - Brazil

^[4] Univ Calif San Diego, Dept Pediat, San Diego, CA 92103 - USA

^[5] Univ Calif San Diego, Ctr Microbiome Innovat, San Diego, CA 92103 - USA

• Tipo de documento: Artigo de Revisão
• Fonte: NATURE REVIEWS MICROBIOLOGY; v. 20, n. 3 SEP 2021.
• Citações Web of Science: 3

Resumo

Mass spectrometry-based metabolomics is one of the key technologies to detect and identify the small molecules produced by the human microbiota and to understand the functional role of these microbial metabolites. In this Review, Dorrestein and colleagues review common forms of untargeted mass spectrometry and the types of data that can be obtained in the context of microbiome analysis. Microbiotas are a malleable part of ecosystems, including the human ecosystem. Microorganisms affect not only the chemistry of their specific niche, such as the human gut, but also the chemistry of distant environments, such as other parts of the body. Mass spectrometry-based metabolomics is one of the key technologies to detect and identify the small molecules produced by the human microbiota, and to understand the functional role of these microbial metabolites. This Review provides a foundational introduction to common forms of untargeted mass spectrometry and the types of data that can be obtained in the context of microbiome analysis. Data analysis remains an obstacle; therefore, the emphasis is placed on data analysis approaches and integrative analysis, including the integration of microbiome sequencing data. (AU)

• Processo FAPESP: 15/17177-6 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 18/24865-4 - Novas abordagens em metabolomica: Cartografia Molecular 3D de ascídias baseado em dados de LC-MS/MS
• Beneficiário: Anelize Bauermeister
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 18/07098-0 - Efeito de fármacos marinhos em Peixe Zebra e na linhagem ZFL - emphasis
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Regular