In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery

Autor(es)

de Souza, Jaqueline Brandao ^[1] ; Rosa, Gustavo dos Santos ^[1] ; Rossi, Mariana Correa ^[1] ; Stievani, Fernanda de Castro ^[1] ; Pfeifer, Joao Pedro Hubbe ^[1] ; Teramoto Krieck, Andre Massahiro ^[1] ; de Carvalho Bovolato, Ana Livia ^[2] ; Fonseca-Alves, Carlos Eduardo ^{[1, 3]} ; Amigo Borras, Vicente ^[4] ; Garcia Alves, Ana Liz ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Surg & Anim Reprod, UNESP, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Blood Transfus Ctr, UNESP, Cell Engn Lab, Botucatu Med Sch, Botucatu, SP - Brazil

^[3] Paulista Univ UNIP Bauru, Inst Hlth Sci, Bauru, SP - Brazil

^[4] Univ Politecn Valencia, Inst Tecnol Mat, Valencia - Spain

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 9, AUG 27 2021.
• Citações Web of Science: 0

Resumo

Encapsulation of biological components in hydrogels is a well described method for controlled drug delivery of proteins, tissue engineering and intestinal colonization with beneficial bacteria. Given the potential of tissue engineering in clinical practice, this study aimed to evaluate the feasibility of encapsulation of adipose tissue-derived mesenchymal stem cells (MSCs) of mules in sodium alginate. We evaluated capsule morphology and cell viability, immunophenotype and release after encapsulation. Circular and irregular pores were observed on the hydrogel surface, in which MSCs were present and alive. Capsules demonstrated good capacity of absorption of liquid and cell viability was consistently high through the time points, indicating proper nutrient diffusion. Flow cytometry showed stability of stem cell surface markers, whereas immunohistochemistry revealed the expression of CD44 and absence of MHC-II through 7 days of culture. Stem cell encapsulation in sodium alginate hydrogel is a feasible technique that does not compromise cell viability and preserves their undifferentiated status, becoming a relevant option to further studies of tridimensional culture systems and in vivo bioactive agents delivery.</p> (AU)

• Processo FAPESP: 17/01369-9 - Tratamento da sinovite em equinos: efeito das células-tronco mesenquimais envoltas por microcápsulas sobre os mediadores inflamatórios articulares
• Beneficiário: Gustavo dos Santos Rosa
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/12815-0 - Dor e qualidade de vida em animais
• Beneficiário: Stelio Pacca Loureiro Luna
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/14460-4 - Efeito da administração intra-articular de microcápsulas com células tronco mesenquimais adultas no tratamento da osteoartrite
• Beneficiário: Ana Liz Garcia Alves
• Modalidade de apoio: Auxílio à Pesquisa - Regular