| Full text | |
| Author(s): |
de Souza, Jaqueline Brandao
[1]
;
Rosa, Gustavo dos Santos
[1]
;
Rossi, Mariana Correa
[1]
;
Stievani, Fernanda de Castro
[1]
;
Pfeifer, Joao Pedro Hubbe
[1]
;
Teramoto Krieck, Andre Massahiro
[1]
;
de Carvalho Bovolato, Ana Livia
[2]
;
Fonseca-Alves, Carlos Eduardo
[1, 3]
;
Amigo Borras, Vicente
[4]
;
Garcia Alves, Ana Liz
[1]
Total Authors: 10
|
| Affiliation: | [1] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Surg & Anim Reprod, UNESP, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Blood Transfus Ctr, UNESP, Cell Engn Lab, Botucatu Med Sch, Botucatu, SP - Brazil
[3] Paulista Univ UNIP Bauru, Inst Hlth Sci, Bauru, SP - Brazil
[4] Univ Politecn Valencia, Inst Tecnol Mat, Valencia - Spain
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 9, AUG 27 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Encapsulation of biological components in hydrogels is a well described method for controlled drug delivery of proteins, tissue engineering and intestinal colonization with beneficial bacteria. Given the potential of tissue engineering in clinical practice, this study aimed to evaluate the feasibility of encapsulation of adipose tissue-derived mesenchymal stem cells (MSCs) of mules in sodium alginate. We evaluated capsule morphology and cell viability, immunophenotype and release after encapsulation. Circular and irregular pores were observed on the hydrogel surface, in which MSCs were present and alive. Capsules demonstrated good capacity of absorption of liquid and cell viability was consistently high through the time points, indicating proper nutrient diffusion. Flow cytometry showed stability of stem cell surface markers, whereas immunohistochemistry revealed the expression of CD44 and absence of MHC-II through 7 days of culture. Stem cell encapsulation in sodium alginate hydrogel is a feasible technique that does not compromise cell viability and preserves their undifferentiated status, becoming a relevant option to further studies of tridimensional culture systems and in vivo bioactive agents delivery.</p> (AU) | |
| FAPESP's process: | 17/01369-9 - Treatment of synovitis in horses: effect of mesenchymal stem cells involved by microcapsules on the articular inflammatory mediators |
| Grantee: | Gustavo dos Santos Rosa |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 17/12815-0 - Pain and quality of life in animals |
| Grantee: | Stelio Pacca Loureiro Luna |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/14460-4 - EFFECT OF INTRAARTICULAR INJECTION OF MICROCAPSULES WITH MESENCHYMAL STEM CELLS ON THE TREATMENT OF OSTEOARTHRITIS |
| Grantee: | Ana Liz Garcia Alves |
| Support Opportunities: | Regular Research Grants |