Corona protein impacts on alternating current biosusceptometry signal and circulation times of differently coated MnFe2O4 nanoparticles

Autor(es)

Prospero, Andre Goncalves ^[1] ; Buranello, Lais Pereira ^[1] ; Fernandes, Carlos A. H. ^{[1, 2]} ; dos Santos, Lucilene Delazari ^{[3, 4]} ; Soares, Guilherme ^[1] ; Rossini, Bruno C. ^[3] ; Zufelato, Nicholas ^{[5, 6]} ; Bakuzis, Andris Figueiroa ^{[5, 6]} ; de Mattos Fontes, Marcos R. ^[1] ; de Arruda Miranda, Jose R. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Biophys & Pharmacol, BR-18618689 Botucatu, SP - Brazil

^[2] Sorbonne Univ, Inst Mineral Phys Mat & Cosmochim, CNRS, IMPMC, UMR 7590, Museum Natl Hist Nat, F-7590 Paris - France

^[3] Sao Paulo State Univ, Biotechnol Inst, BR-18607440 Botucatu, SP - Brazil

^[4] Sao Paulo State Univ, Botucatu Med Sch FMB, Grad Program Trop Dis, UNESP, BR-18618687 Botucatu, SP - Brazil

^[5] Univ Fed Goias, Inst Phys, BR-74690900 Goiania, Go - Brazil

^[6] Univ Fed Goias, CNanoMed, BR-74690900 Goiania, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Nanomedicine; v. 16, n. 24 SEP 2021.
• Citações Web of Science: 0

Resumo

Background: We evaluated the impacts of corona protein (CP) formation on the alternating current biosusceptometry (ACB) signal intensity and in vivo circulation times of three differently coated magnetic nanoparticles (MNP): bare, citrate-coated and bovine serum albumin-coated MNPs. Methods: We employed the ACB system, gel electrophoresis and mass spectrometry analysis. Results: Higher CP formation led to a greater reduction in the in vitro ACB signal intensity and circulation time. We found fewer proteins forming the CP for the bovine serum albumin-coated MNPs, which presented the highest circulation time in vivo among the MNPs studied. Conclusion: These data showed better biocompatibility, stability and magnetic signal uniformity in biological media for bovine serum albumin-coated MNPs than for citrate-coated MNPs and bare MNPs. (AU)

• Processo FAPESP: 15/14923-9 - Instrumentação e aplicações por biosusceptometria de corrente alternada: estudos de distribuição de nanopartículas, motilidade e controle da qualidade de formas farmacêuticas sólidas
• Beneficiário: José Ricardo de Arruda Miranda
• Modalidade de apoio: Auxílio à Pesquisa - Regular