Electrophilic oxysterols: generation, measurement and protein modification

Autor(es)

Miyamoto, Sayuri ^[1] ; Lima, Rodrigo S. ^[1] ; Inague, Alex ^[1] ; Viviani, Lucas G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim, Inst Quim, CP 26077, BR-05513970 Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Free Radical Research; v. 55, n. 4, SI, p. 416-440, APR 3 2021.
• Citações Web of Science: 2

Resumo

Cholesterol is an essential component of mammalian plasma membranes. Alterations in sterol metabolism or oxidation have been linked to various pathological conditions, including cardiovascular diseases, cancer, and neurodegenerative disorders. Unsaturated sterols are vulnerable to oxidation induced by singlet oxygen and other reactive oxygen species. This process yields reactive sterol oxidation products, including hydroperoxides, epoxides as well as aldehydes. These oxysterols, in particular those with high electrophilicity, can modify nucleophilic sites in biomolecules and affect many cellular functions. Here, we review the generation and measurement of reactive sterol oxidation products with emphasis on cholesterol hydroperoxides and aldehyde derivatives (electrophilic oxysterols) and their effects on protein modifications. (AU)

• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/13804-1 - Mecanismos de detoxificação e reparo de membranas biológicas oxidadas envolvendo a ação da enzima peroxirredoxina 6
• Beneficiário: Alex Inague
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/50891-0 - Ácido docosahexaenóico e colesterol: caracterização e detecção de produtos de oxidação, modificação de proteínas e implicações na esclerose lateral amiotrófica
• Beneficiário: Sayuri Miyamoto
• Modalidade de apoio: Auxílio à Pesquisa - Regular