Modification of zeolite with metallic ions improves the immobilization of phytase

Autor(es)

Lopes, M. M. ^{[1, 2]} ; Coutinho, T. C. ^{[3, 1]} ; Farinas, C. S. ^{[3, 1, 2]}

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentat, Rua XV Novembro, 1452, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Grad Program Biotechnol, BR-13560000 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 36, SEP 2021.
• Citações Web of Science: 0

Resumo

Phytase is an enzyme applied in several fields, but its wider use is still limited due to its high cost and poor stability, which can be overcome using immobilization techniques. Zeolites are promising support materials for enzyme immobilization, due to their attractive properties, including the capacity for modification using a simple ion exchange procedure, enabling better chemical interaction with proteins. In this work, investigation was made of the immobilization of phytase onto zeolites modified with metallic ions (Ag, Cd, Zn, Cu, Ca, Mg, and Ni). The zeolite modified with nickel reached immobilization yield of 66%, while the recovered activity (150%) indicated activation of the catalytic activity due to the presence of the metallic ion. The immobilized phytase showed excellent performance under conditions of acidic pH, reaching a relative activity up to 7 times higher than observed for its free form. Furthermore, it was possible to recover and reuse effectively the zeolite-Ni/Phytase derivative in at least 4 consecutive cycles of phytate hydrolysis reaction. The phytase immobilized onto zeolite modified with nickel presented improved properties, expanding the range of potential applications of this important enzyme. These findings demonstrate an efficient and simple protocol for enzyme immobilization onto zeolites modified with metallic ions. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático