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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Modification of zeolite with metallic ions improves the immobilization of phytase

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Author(s):
Lopes, M. M. [1, 2] ; Coutinho, T. C. [3, 1] ; Farinas, C. S. [3, 1, 2]
Total Authors: 3
Affiliation:
[1] Embrapa Instrumentat, Rua XV Novembro, 1452, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Grad Program Biotechnol, BR-13560000 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 36, SEP 2021.
Web of Science Citations: 0
Abstract

Phytase is an enzyme applied in several fields, but its wider use is still limited due to its high cost and poor stability, which can be overcome using immobilization techniques. Zeolites are promising support materials for enzyme immobilization, due to their attractive properties, including the capacity for modification using a simple ion exchange procedure, enabling better chemical interaction with proteins. In this work, investigation was made of the immobilization of phytase onto zeolites modified with metallic ions (Ag, Cd, Zn, Cu, Ca, Mg, and Ni). The zeolite modified with nickel reached immobilization yield of 66%, while the recovered activity (150%) indicated activation of the catalytic activity due to the presence of the metallic ion. The immobilized phytase showed excellent performance under conditions of acidic pH, reaching a relative activity up to 7 times higher than observed for its free form. Furthermore, it was possible to recover and reuse effectively the zeolite-Ni/Phytase derivative in at least 4 consecutive cycles of phytate hydrolysis reaction. The phytase immobilized onto zeolite modified with nickel presented improved properties, expanding the range of potential applications of this important enzyme. These findings demonstrate an efficient and simple protocol for enzyme immobilization onto zeolites modified with metallic ions. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants