Lifelong Adaptation of Gastric Cell Proliferation and Mucosa Structure to Early Weaning-Induced Effects

Autor(es)

Mesquita da Silva, Kethleen ^[1] ; Rattes, Isadora Campos ^[1] ; Pereira, Gizela Maria Agostini ^[1] ; Gama, Patricia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PHYSIOLOGY; v. 12, SEP 13 2021.
• Citações Web of Science: 0

Resumo

The gastric mucosa is disturbed when breastfeeding is interrupted, and such early weaning (EW) condition permanently affects the differentiation of zymogenic cells. The aim of the study was to evaluate the immediate and long-term effects of EW on gastric cell proliferation, considering the molecular markers for cell cycle, inflammation, and metaplasia. Overall, we investigated the lifelong adaptation of gastric growth. Wistar rats were divided into suckling-control (S) and EW groups, and gastric samples were collected at 18, 30, and 60 days for morphology, RNA, and protein isolation. Inflammation and metaplasia were not identified, but we observed that EW promptly increased Ki-67-proliferative index (PI) and mucosa thickness (18 days). From 18 to 30 days, PI increased in S rats, whereas it was stable in EW animals, and such developmental change in S made its PI higher than in EW. At 60 days, the PI decreased in S, making the indices similar between groups. Spatially, during development, proliferative cells spread along the gland, whereas, in adults, they concentrate at the isthmus-neck area. EW pushed dividing cells to this compartment (18 days), increased PI at the gland base (60 days), but it did not interfere in expression of cell cycle molecules. At 18 days, EW reduced Tgf beta 2, Tgf beta 3, and Tgfbr2 and T beta RII and p27 levels, which might regulate the proliferative increase at this age. We demonstrated that gastric cell proliferation is immediately upregulated by EW, corroborating previous results, but for the first time, we showed that such increased PI is stable during growth and aging. We suggest that suckling and early weaning might use TGF beta s and p27 to trigger different proliferative profiles during life course.</p> (AU)

• Processo FAPESP: 14/21449-9 - Expressão gênica, diferenciação celular e funções gástricas: distúrbios alimentares durante o desenvolvimento pós-natal podem alterar o programa de crescimento em ratos?
• Beneficiário: Patrícia Gama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/05064-0 - Caracterização do nicho de células-tronco na mucosa gástrica de ratos em desenvolvimento e adultos e o papel do desmame precoce sobre essa população
• Beneficiário: Isadora Campos Rattes
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/07782-8 - Efeito do desmame precoce sobre marcadores moleculares durante o crescimento e desenvolvimento pós- natal da mucosa intestinal do rato.
• Beneficiário: Patrícia Gama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/09522-6 - Adaptações duradouras da proliferação celular e da estrutura da mcuosa gástrica a efeitos induzidos pelo desmame precoce
• Beneficiário: Patrícia Gama
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo