| Full text | |
| Author(s): |
Mesquita da Silva, Kethleen
[1]
;
Rattes, Isadora Campos
[1]
;
Pereira, Gizela Maria Agostini
[1]
;
Gama, Patricia
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PHYSIOLOGY; v. 12, SEP 13 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
The gastric mucosa is disturbed when breastfeeding is interrupted, and such early weaning (EW) condition permanently affects the differentiation of zymogenic cells. The aim of the study was to evaluate the immediate and long-term effects of EW on gastric cell proliferation, considering the molecular markers for cell cycle, inflammation, and metaplasia. Overall, we investigated the lifelong adaptation of gastric growth. Wistar rats were divided into suckling-control (S) and EW groups, and gastric samples were collected at 18, 30, and 60 days for morphology, RNA, and protein isolation. Inflammation and metaplasia were not identified, but we observed that EW promptly increased Ki-67-proliferative index (PI) and mucosa thickness (18 days). From 18 to 30 days, PI increased in S rats, whereas it was stable in EW animals, and such developmental change in S made its PI higher than in EW. At 60 days, the PI decreased in S, making the indices similar between groups. Spatially, during development, proliferative cells spread along the gland, whereas, in adults, they concentrate at the isthmus-neck area. EW pushed dividing cells to this compartment (18 days), increased PI at the gland base (60 days), but it did not interfere in expression of cell cycle molecules. At 18 days, EW reduced Tgf beta 2, Tgf beta 3, and Tgfbr2 and T beta RII and p27 levels, which might regulate the proliferative increase at this age. We demonstrated that gastric cell proliferation is immediately upregulated by EW, corroborating previous results, but for the first time, we showed that such increased PI is stable during growth and aging. We suggest that suckling and early weaning might use TGF beta s and p27 to trigger different proliferative profiles during life course.</p> (AU) | |
| FAPESP's process: | 14/21449-9 - Gene expression, cell differentiation and gastric functions: can alimentary changes during postnatal development disturb the growth program in rats? |
| Grantee: | Patrícia Gama |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/05064-0 - Characterization of the stem cell niche in the gastric mucosa of developing and adult rats and the role of early weaning on this population |
| Grantee: | Isadora Campos Rattes |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 18/07782-8 - Effects of early weaning on molecular markers during postnatal growth and development of intestinal mucosa in the rat. |
| Grantee: | Patrícia Gama |
| Support Opportunities: | Regular Research Grants |