| Texto completo | |
| Autor(es): |
da Silveira, Juliano Coelho
[1]
;
Andrade, Gabriella Mamede
[1, 2]
;
Simas, Rosineide Costa
[3]
;
Martins-Junior, Helio Alves
[4]
;
Eberlin, Marcos Nogueira
[5]
;
Smith, Lawrence Charles
[6, 1]
;
Perecin, Felipe
[1]
;
Meirelles, Flavio Vieira
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13600900 Pirassununga, SP - Brazil
[2] Nilo Frantz Reprod Med, Porto Alegre, RS - Brazil
[3] Univ Fed Goias, Inst Chem, Lab Chromatog & Mass Spectrometry, Goiania, Go - Brazil
[4] SCIEX Brazil, Sao Paulo - Brazil
[5] Univ Prebiteriana Mackenzie, Sch Engn PPGEMN, MackMass Lab, Sao Paulo, SP - Brazil
[6] Univ Montreal, Fac Med Vet, Ctr Rech Reprod & Fertilite, St Hyacinthe, PQ - Canada
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Theriogenology; v. 174, p. 1-8, OCT 15 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Cell communication within the ovarian follicle is crucial during folliculogenesis to assure an ideal environment for the oocyte to achieve full developmental competence. Intercellular communication is facilitated by the presence of follicular fluid, which mediates the transfer of signaling molecules. Recently, extracellular vesicles (exosomes and microvesicles) containing mRNAs, miRNAs and proteins were described in mammalian follicular fluid. Besides these molecules, extracellular vesicles (EVs) can mediate the transfer of lipids that can act as signal transducers activating second messengers and modulating intracellular pathways. Our goal was to determine the lipid profile of exosomes (small extracellular vesicles) and microvesicles (large extracellular vesicles) from bovine ovarian follicles con-taining oocytes with different developmental capabilities to verify potential relationships to competence. Using mass spectrometry, we examined the lipid content of EVs present in the follicular fluid of follicles enclosing oocytes that were either unable to cleave (NCLEAVE), arrested at cleavage stage (CLEAVE), or developed to the blastocyst stage (BLAST) after parthenogenetic activation. Although most of the 514 lipids identified in the follicular fluid EVs were common among all groups, 10 exosome-derived lipids and 15 microvesicle-derived lipids were present exclusively in the BLAST group, suggesting a potential relationship with developmental competence. Therefore, our data indicate that the EVs present in follicular fluid of antral follicles of similar morphology contain lipids that may be used as biomarkers associated with the developmental capability of the oocyte to develop to the blastocyst stage. (c) 2021 Elsevier Inc. All rights reserved. (AU) | |
| Processo FAPESP: | 11/15416-2 - Prospecção retrospectiva de lipídios presentes no líquido folicular associados ao potencial de desenvolvimento dos oócitos. |
| Beneficiário: | Gabriella Mamede Andrade |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 19/15491-6 - Mecanismo de ação dos efetores Hippo na regulação da cascata de eventos pré-ovulatórios em bovinos |
| Beneficiário: | Flávio Vieira Meirelles |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 15/21829-9 - EMU concedido no projeto 2014/22887-0: NanoSight LM10 |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 12/50533-2 - GIFT: melhoramento genômico de características relacionadas com a fertilização em gado bovino dinamarquês e brasileiro |
| Beneficiário: | Marcelo Fábio Gouveia Nogueira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/08135-2 - CTC - Centro de Terapia Celular |
| Beneficiário: | Dimas Tadeu Covas |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 14/21034-3 - Rotas intracelulares e intercelulares durante a foliculogênese e maturação oocitária in vivo e in vitro |
| Beneficiário: | Felipe Perecin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |