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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lipid profile of extracellular vesicles and their relationship with bovine oocyte developmental competence: New players in intra follicular cell communication

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Author(s):
da Silveira, Juliano Coelho [1] ; Andrade, Gabriella Mamede [1, 2] ; Simas, Rosineide Costa [3] ; Martins-Junior, Helio Alves [4] ; Eberlin, Marcos Nogueira [5] ; Smith, Lawrence Charles [6, 1] ; Perecin, Felipe [1] ; Meirelles, Flavio Vieira [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13600900 Pirassununga, SP - Brazil
[2] Nilo Frantz Reprod Med, Porto Alegre, RS - Brazil
[3] Univ Fed Goias, Inst Chem, Lab Chromatog & Mass Spectrometry, Goiania, Go - Brazil
[4] SCIEX Brazil, Sao Paulo - Brazil
[5] Univ Prebiteriana Mackenzie, Sch Engn PPGEMN, MackMass Lab, Sao Paulo, SP - Brazil
[6] Univ Montreal, Fac Med Vet, Ctr Rech Reprod & Fertilite, St Hyacinthe, PQ - Canada
Total Affiliations: 6
Document type: Journal article
Source: Theriogenology; v. 174, p. 1-8, OCT 15 2021.
Web of Science Citations: 0
Abstract

Cell communication within the ovarian follicle is crucial during folliculogenesis to assure an ideal environment for the oocyte to achieve full developmental competence. Intercellular communication is facilitated by the presence of follicular fluid, which mediates the transfer of signaling molecules. Recently, extracellular vesicles (exosomes and microvesicles) containing mRNAs, miRNAs and proteins were described in mammalian follicular fluid. Besides these molecules, extracellular vesicles (EVs) can mediate the transfer of lipids that can act as signal transducers activating second messengers and modulating intracellular pathways. Our goal was to determine the lipid profile of exosomes (small extracellular vesicles) and microvesicles (large extracellular vesicles) from bovine ovarian follicles con-taining oocytes with different developmental capabilities to verify potential relationships to competence. Using mass spectrometry, we examined the lipid content of EVs present in the follicular fluid of follicles enclosing oocytes that were either unable to cleave (NCLEAVE), arrested at cleavage stage (CLEAVE), or developed to the blastocyst stage (BLAST) after parthenogenetic activation. Although most of the 514 lipids identified in the follicular fluid EVs were common among all groups, 10 exosome-derived lipids and 15 microvesicle-derived lipids were present exclusively in the BLAST group, suggesting a potential relationship with developmental competence. Therefore, our data indicate that the EVs present in follicular fluid of antral follicles of similar morphology contain lipids that may be used as biomarkers associated with the developmental capability of the oocyte to develop to the blastocyst stage. (c) 2021 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/15416-2 - Retrospective prospecting of lipids present in follicular fluid associated with the development potential of oocytes.
Grantee:Gabriella Mamede Andrade
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 19/15491-6 - Mechanism of action of Hippo effectors in the regulation of the cascade of pre-ovulatory events in cattle
Grantee:Flávio Vieira Meirelles
Support Opportunities: Regular Research Grants
FAPESP's process: 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10
Grantee:Juliano Coelho da Silveira
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation
Grantee:Felipe Perecin
Support Opportunities: Regular Research Grants