Characterization of allelochemicals from Pistia stratiotes extracts and their effects on the growth and physiology of Microcystis aeruginosa

Autor(es)

Lourencao, Anderson ^[1] ; Mecina, Gustavo F. ^[1] ; Cordeiro-Araujo, Micheline K. ^[2] ; Bittencourt-Oliveira, Maria C. ^[2] ; Chia, Mathias A. ^[3] ; Bronzel-Junior, Joao L. ^[1] ; Granero, Filipe O. ^[1] ; Silva, Luciana P. ^[4] ; da Silva, Regildo M. G. ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Biol Sci, Piracicaba, SP - Brazil

^[3] Ahmadu Bello Univ, Dept Bot, Zaria - Nigeria

^[4] Fundacao Educ Municipio Assis FEMA, Assis, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, Dept Biotechnol, Lab Herbal Med & Nat Prod, Dom Antonio Ave 2100, BR-19806900 Assis, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 28, n. 40, p. 57248-57259, OCT 2021.
• Citações Web of Science: 0

Resumo

Due to the public and environmental health impact of cyanotoxins, investigations have been focused on finding environmental friendly algaecides from aquatic plants. The present study had the objective to evaluate the population control and physiological response of Microcystis aeruginosa (Kutzing) Kutzing (strain BCCUSP232) exposed to Pistia stratiotes L. extracts. Aqueous and ethanolic extracts of P. stratiotes at different concentrations (10, 25, and 50 mg L-1) were submitted to M. aeruginosa and reduced significantly (p<0.05) the cyanobacterium cell density. The ethanolic extract presented the greatest growth inhibition of the strain at the highest concentration. During exposure to P. stratiotes extracts, intracellular hydrogen peroxide levels, malondialdehyde content, and antioxidant enzymes (peroxidase, catalase, and glutathione S-transferase) activities increased in M. aeruginosa, while total protein concentration decreased when compared to the control group. Superoxide dismutase (SOD) activities presented a sharp decline, suggesting superoxide radical and peroxide accumulation. This implied that SOD was a target for bioactive substance(s) from aqueous and ethanolic extracts of P. stratiotes. Phytochemical screening of the extracts revealed that the ethanolic extract presented 93.36 mg gallic acid equivalent (GAE) per gram dry weight (g(-1) DW) total polyphenols and 217.33 mg rutin equivalent (RE) per gram dry weight total flavonoids, and for the aqueous extract, 5.19 mg GAE g(-1) DW total polyphenols and 11.02 mg RE g(-1) DW total flavonoids were detected. Gas chromatography (GC)/mass spectrometry (MS) analyses of the ethanolic and aqueous extracts presented palmitic acid ethyl ester as major allelochemical. In view of these results, it can be concluded that P. stratiotes showed potential in controlling M. aeruginosa populations. (AU)

• Processo FAPESP: 16/01287-0 - Caracterização de compostos de Hordeum vulgare L., Pistia stratiotes L. e Tridax procumbens L. para o desenvolvimento de método alternativo no controle de cianobactérias produtoras de cianotoxinas
• Beneficiário: Regildo Márcio Gonçalves da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular