The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction

Autor(es)

Pieretti, Joana C. ^[1] ; Beurton, Jordan ^[2] ; Munevar, Julian ^[1] ; Nagamine, Luiz C. C. M. ^[3] ; Le Faou, Alain ^[2] ; Seabra, Amedea B. ^[1] ; Clarot, Igor ^[2] ; Boudier, Ariane ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ ABC UFABC, Ctr Nat & Human Sci CCNH, BR-09210580 Santo Andre, SP - Brazil

^[2] Univ Lorraine, CITHEFOR, F-54000 Nancy - France

^[3] Univ Sao Paulo, Inst Fis, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 19 OCT 2021.
• Citações Web of Science: 0

Resumo

In nanomedicine, hybrid nanomaterials stand out for providing new insights in both the diagnosis and treatment of several diseases. Once administered, engineered nanoparticles (NPs) interact with biological molecules, and the nature of this interaction might directly interfere with the biological fate and action of the NPs. In this work, we synthesized a hybrid magnetic nanostructure, with antibacterial and antitumoral potential applications, composed of a magnetite core covered by silver NPs, and coated with a modified chitosan polymer. As magnetite NPs readily oxidize to maghemite, we investigated the structural properties of the NPs after addition of the two successive layers using Mossbauer spectroscopy. Then, the structural characteristics of the NPs were correlated to their interaction with albumin, the major blood protein, to evidence the consequences of its binding on NP properties and protein retention. Thermodynamic parameters of the NPs-albumin interaction were determined. We observed that the more stable NPs (coated with modified chitosan) present a lower affinity for albumin in comparison to pure magnetite and magnetite/silver hybrid NPs. Surface properties were key players at the NP-biological interface. To the best of our knowledge, this is the first study that demonstrates a correlation between the structural properties of complex hybrid NPs and their interaction with albumin.</p> (AU)

• Processo FAPESP: 18/02832-7 - Nanopartículas híbridas magnéticas e antibacterianas: síntese, caracterização e aplicações
• Beneficiário: Joana Claudio Pieretti
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/07766-5 - Hemocompatibilidade de nanopartículas híbridas de magnetita/prata recobertas com doadores de óxido nítrico
• Beneficiário: Joana Claudio Pieretti
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado