ioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatc

Autor(es)

Camargo, Tavani R. ^[1] ; Mantoan, Paulo ^[1] ; Ramos, Patricia ^[2] ; Monserrat, Jose M. ^{[3, 2]} ; Prentice, Carlos ^[2] ; Fernandes, Celio C. ^[4] ; Zambuzzi, Willian F. ^[4] ; Valenti, Wagner C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Aquaculture Ctr, Campus Jaboticabal, BR-14884900 Jaboticabal, SP - Brazil

^[2] Fed Univ Rio Grande FURG Rio Grande, Oceanog Inst, Marine Stn Aquaculture, Rio Grande, RS - Brazil

^[3] Fed Univ Rio Grande FURG, Inst Biol Sci ICB, Rio Grande, RS - Brazil

^[4] Sao Paulo State Univ UNESP, Biosci Inst, Dept Chem & Biol Sci, Campus Botucatu, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE BIOTECHNOLOGY; v. 23, n. 6 OCT 2021.
• Citações Web of Science: 0

Resumo

The animals from bycatch of the shrimp fisheries can be a source of natural products and bioactive compounds. Thus, the present study aimed to evaluate the bioactivity of protein hydrolysates prepared from the two most abundant crabs from the bycatch of shrimp fisheries in Brazil (Callinectes ornatus and Hepatus pudibundus). Samples of C. ornatus and H. pudibundus were collected in the region of Ubatuba, State of Sao Paulo, Brazil. Muscles with small pieces of exoskeleton of both species were hydrolyzed using two enzymes, Alcalase 2.4 L (R) or Protamex (R). The in vitro antioxidant capacity was analyzed used three methods: DPPH, sulfhydryl groups, and peroxyl radicals. Additionally, the cytotoxicity of the hydrolysates was investigated using pre-osteoblasts cells. The results showed that the degree of hydrolysis (DH) of H. pudibundus was superior to DH of C. ornatus using both enzymes and was higher when using the enzyme Alcalase 2.4 L (R) (32.0% +/- 1.9). The analysis suggested that the hydrolysates have antioxidant activity. Besides that, no cytotoxic effect was observed on cell viability. Thus, protein hydrolysates of C. ornatus and H. pudibundus have bioactivity, which add value to these bycatch species and suggests their potential use as nutraceutical ingredient in the food industry. (AU)

• Processo FAPESP: 18/18995-2 - Atividade antimicrobiana e microencapsulação de peptídeos bioativos obtidos a partir de hidrolisados proteicos da fauna acompanhante da pesca camaroeira
• Beneficiário: Tavani Rocha Camargo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/11182-0 - Bioprospecção de compostos antioxidantes na fauna acompanhante da pesca demersal e uso dessas biomoléculas como nutracêuticos na aquicultura
• Beneficiário: Tavani Rocha Camargo
• Modalidade de apoio: Bolsas no Brasil - Doutorado