Effects of imidacloprid on viability and increase of reactive oxygen and nitrogen species in HepG2 cell line

Autor(es)

de Jesus Santos Guimaraes, Anilda Rufino ^[1] ; Veiga Bizerra, Paulo Francisco ^{[1, 2]} ; Miranda, Camila Araujo ^[1] ; Mingatto, Fabio Erminio ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Coll Agr & Technol Sci, Dept Anim Sci, BR-17900000 Dracena, SP - Brazil

^[2] Maringa State Univ UEM, Dept Biochem, Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY MECHANISMS AND METHODS; OCT 2021.
• Citações Web of Science: 0

Resumo

Imidacloprid (IMD) is a neonicotinoid insecticide used in large quantities worldwide in both veterinary and agronomic applications. Several studies have shown adverse effects of IMD on non-target organisms, with the liver being identified as the main affected organ. This study aimed to evaluate the effects of IMD on human hepatoblastoma (HepG2) cells. HepG2 were exposed to IMD (0.25-2.0 mM) for 24 and 48 h. IMD treatment resulted in cytotoxicity in the HepG2, inhibiting cell proliferation in a dose- and time-dependent manner, starting at concentrations of 0.5 mM (24 h) and 0.25 mM (48 h), and reducing cell viability from 0.5 mM onwards (24 and 48 h). IMD significantly decreased the mitochondrial membrane potential at both time points investigated (2.0 mM), and also induced damage to the cell membrane, demonstrated by significant dose and time-dependent increases in lactate dehydrogenase (LDH) release from concentrations of 1.0 mM (24 h) and 0.5 mM (48 h) upwards. IMD treatment also increased the production of reactive oxygen and nitrogen species (ROS/RNS) at rates above 50% following 0.5 mM (24 h) or 0.25 mM (48 h) concentrations, and caused a significant decrease in reduced/oxidized glutathione ratio (GSH/GSSG), indicating oxidative stress. Furthermore, the antioxidant dithiothreitol, which reacts with ROS/RNS and acts as a thiol reducing agent, inhibited the cytotoxic effect of IMD. In addition, the metabolite IMD-olefin was more toxic than IMD. Our results indicate that IMD induces cytotoxicity in HepG2 cells and that this effect may be associated with an increase in the generation of ROS/RNS. (AU)

• Processo FAPESP: 15/19549-8 - Mecanismos de toxicidade do inseticida imidacloprido e dos seus metabólitos desnitro-imidacloprido e imidacloprido-olefina em mitocôndrias e hepatócitos primários isolados de rato e células HepG2
• Beneficiário: Fábio Erminio Mingatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular