M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment

Autor(es)

Raquel Assed Bezerra Da Silva ^[1] ; Léa Assed Bezerra Da Silva ^[2] ; Edson Alves Gabriel-Junior ^[3] ; Carlos Arterio Sorgi ^[4] ; Lúcia Helena Faccioli ^[5] ; Vivian Vicentin Massoni ^[6] ; Paulo Nelson-Filho ^[7] ; Carolina Maschietto Pucinelli ^[8]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil

^[2] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil

^[3] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas - Brasil

^[4] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto - Brasil

^[5] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas - Brasil

^[6] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil

^[7] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil

^[8] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 32, n. 3, p. 32-43, 2021-11-08.

Resumo

Abstract The aim of this study was to evaluate the M1 and M2 macrophage modulation after stimuli with different materials used during endodontic treatment. In bone marrow-derived macrophage cell culture, from males C57BL/6 wild-type (WT) mice, gene expression analysis of markers to M1 and M2 macrophages was performed by qRT-PCR (Cxcl10, CxCL9, iNOS, Arg1, Chil3, Retnla and MRC1) and cytokine quantification by Luminex® (GM-CSF, IL-10, IL-6, IL-1β and TNF-α) after exposure to the five endodontic sealers: AH Plus, Sealapex Xpress, Endosequence BC Sealer, BioRoot RCS and a calcium hydroxide-based paste. For normal values, ANOVA test was used, followed by Tukey post-test. For non-normal values, the Kruskall-Wallis test was used. BioRootTM RCS and EndoSequence BC SealerTM stimulated the highest expression of markers for M1 macrophages, while calcium hydroxide-based paste stimulated the lowest expression of these gene markers. For M2 protein markers, BioRootTM RCS presented the highest stimulation while calcium hydroxide-based paste also presented the lowest stimulation. It was concluded that all the evaluated filling materials increased the genetic expression of pro- and anti-inflammatory markers: TNF-α and IL-10 respectively. The others proinflammatory mediators showed differences against the filling materials. However, this process did not induce the inflammatory response polarization, resulting in a hybrid macrophage. (AU)

• Processo FAPESP: 16/24900-9 - Participação dos macrófagos M1 e M2 na gênese, desenvolvimento e tratamento da lesão periapical
• Beneficiário: Paulo Nelson Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular