Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment

Full text
Author(s):
Raquel Assed Bezerra Da Silva [1] ; Léa Assed Bezerra Da Silva [2] ; Edson Alves Gabriel-Junior [3] ; Carlos Arterio Sorgi [4] ; Lúcia Helena Faccioli [5] ; Vivian Vicentin Massoni [6] ; Paulo Nelson-Filho [7] ; Carolina Maschietto Pucinelli [8]
Total Authors: 8
Affiliation:
[1] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil
[2] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil
[3] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas - Brasil
[4] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto - Brasil
[5] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas - Brasil
[6] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil
[7] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil
[8] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Departamento de Clínica Infantil - Brasil
Total Affiliations: 8
Document type: Journal article
Source: Brazilian Dental Journal; v. 32, n. 3, p. 32-43, 2021-11-08.
Abstract

Abstract The aim of this study was to evaluate the M1 and M2 macrophage modulation after stimuli with different materials used during endodontic treatment. In bone marrow-derived macrophage cell culture, from males C57BL/6 wild-type (WT) mice, gene expression analysis of markers to M1 and M2 macrophages was performed by qRT-PCR (Cxcl10, CxCL9, iNOS, Arg1, Chil3, Retnla and MRC1) and cytokine quantification by Luminex® (GM-CSF, IL-10, IL-6, IL-1β and TNF-α) after exposure to the five endodontic sealers: AH Plus, Sealapex Xpress, Endosequence BC Sealer, BioRoot RCS and a calcium hydroxide-based paste. For normal values, ANOVA test was used, followed by Tukey post-test. For non-normal values, the Kruskall-Wallis test was used. BioRootTM RCS and EndoSequence BC SealerTM stimulated the highest expression of markers for M1 macrophages, while calcium hydroxide-based paste stimulated the lowest expression of these gene markers. For M2 protein markers, BioRootTM RCS presented the highest stimulation while calcium hydroxide-based paste also presented the lowest stimulation. It was concluded that all the evaluated filling materials increased the genetic expression of pro- and anti-inflammatory markers: TNF-α and IL-10 respectively. The others proinflammatory mediators showed differences against the filling materials. However, this process did not induce the inflammatory response polarization, resulting in a hybrid macrophage. (AU)

FAPESP's process: 16/24900-9 - Participation of macrophages M1 and M2 in the genesis, development and treatment of periodical lesions
Grantee:Paulo Nelson Filho
Support Opportunities: Regular Research Grants