Restricted feeding modulates peripheral clocks and nutrient sensing pathways in rats

Autor(es)

Luis Guilherme F. Rodrigues ^[1] ; Leonardo D. de Araujo ^[2] ; Silvia L. R. Roa ^[3] ; Ana C. Bueno ^[4] ; Ernane T. Uchoa ^[5] ; José Antunes-Rodrigues ^[6] ; Ayrton C. Moreira ^[7] ; Lucila L. K. Elias ^[8] ; Margaret de Castro ^[9] ; Clarissa S. Martins ^[10]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Medicina Interna - Brasil

^[2] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Fisiologia - Brasil

^[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Medicina Interna - Brasil

^[4] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Pediatria - Brasil

^[5] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Fisiologia - Brasil

^[6] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Fisiologia - Brasil

^[7] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Medicina Interna - Brasil

^[8] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Fisiologia - Brasil

^[9] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Medicina Interna - Brasil

^[10] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Medicina Interna - Brasil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ENDOCRINOLOGY METABOLISM; v. 65, n. 5, p. 549-561, 2021-09-29.

Resumo

ABSTRACT Objective: Feeding restriction in rats alters the oscillators in suprachiasmatic, paraventricular, and arcuate nuclei, hypothalamic areas involved in food intake. In the present study, using the same animals and experimental protocol, we aimed to analyze if food restriction could reset clock genes ( Clock, Bmal1 ) and genes involved in lipid metabolism ( Pgc1a, Pparg, Ucp2 ) through nutrient-sensing pathways ( Sirt1, Ampk, Nampt ) in peripheral tissues. Materials and methods: Rats were grouped according to food access: Control group (CG, food ad libitum ), Restricted night-fed (RF-n, food access during 2 h at night), Restricted day-fed (RF-d, food access during 2 h in the daytime), and Day-fed (DF, food access during 12 h in the daytime). After 21 days, rats were decapitated at ZT3 (0900-1000 h), ZT11 (1700-1800 h), or ZT17 (2300-2400 h). Blood, liver, brown (BAT) and peri-epididymal (PAT) adipose tissues were collected. Plasma corticosterone and gene expression were evaluated by radioimmunoassay and qPCR, respectively. Results: In the liver, the expression pattern of Clock and Bmal1 shifted when food access was dissociated from rat nocturnal activity; this phenomenon was attenuated in adipose tissues. Daytime feeding also inverted the profile of energy-sensing and lipid metabolism-related genes in the liver, whereas calorie restriction induced a pre-feeding increased expression of these genes. In adipose tissues, Sirt1 expression was modified by daytime feeding and calorie restriction, with concomitant expression of Pgc1a , Pparg , and Ucp2 but not Ampk and Nampt . Conclusion: Feeding restriction reset clock genes and genes involved in lipid metabolism through nutrient-sensing-related genes in rat liver, brown, and peri-epididymal adipose tissues. (AU)

• Processo FAPESP: 12/02234-6 - Análise de genes envolvidos na regulação do ciclo celular e seu controle por proteínas ribossomais na gênese molecular dos adenomas hipofisários
• Beneficiário: Clarissa Silva Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/58365-3 - Fisiopatologia e etiopatogenia molecular de doenças relacionadas aos eixos corticotrófico, somatotrófico e neurohipofisário
• Beneficiário: Margaret de Castro
• Modalidade de apoio: Auxílio à Pesquisa - Temático