ntradermal comparative cervical tuberculin test in the diagnosis of caprine tuberculosi

Autor(es)

Scacchetti de Almeida, Carlos Augusto ^[1] ; dos Santos, Caio R. ^[2] ; Benites, Nilson Roberti ^[3] ; Jordao, Ricardo S. ^[4] ; Chimara, Erica ^[5] ; Benesi, Fernando Jose ^[6] ; de Azevedo, Sergio S. ^[7] ; Ribeiro, Marcio Garcia ^[8] ; Pinheiro, Sonia Regina ^[3]

Afiliação do(s) autor(es)

^[1] Secretariat Agr & Supply State Sao Paulo, Coordinat Sustainable Rural Dev, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Anim Pathol Dept, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Prevent Vet Med & Anim Sci, Sao Paulo, SP - Brazil

^[4] Secretariat Agr & Supply State Sao Paulo, Biol Inst Sao Paulo, Sao Paulo, SP - Brazil

^[5] Adolfo Lutz Inst, TB & Mycobacteriosies Lab, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Dept Clin, Sao Paulo, SP - Brazil

^[7] Univ Fed Campina Grande, Vet Med Acad Unit, UFCG, Campina Grande, Paraiba - Brazil

^[8] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Prod & Prevent Vet Med, UNESP, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 53, n. 1 NOV 2021.
• Citações Web of Science: 0

Resumo

Tuberculosi remains one of the most important infectious diseases with well-known zoonotic nature that affect humans, wildlife, and domestic animals, including goats. Nonetheless, no intradermal tuberculin test has been standardized for caprine diagnosis of tuberculosis. The present study investigated the intradermal comparative cervical tuberculin test (ICCTT) in the diagnosis of tuberculosis among 60 goats from farms with history of tuberculosis. The cutoff applied to goats was based on a study where goats had been experimentally infected with Mycobacterium bovis and Mycobacterium avium. Clinical examination, bacteriological culture, and histopathological staining were assessed to the diagnosis. Isolates compatible with mycobacteria were subjected for molecular diagnosis based on gyrB-restriction fragment length polymorphism (RFLP) analysis and PCR restriction-enzyme analysis (PRA) of hsp65 gene by BstEII and HaeIII, namely PRA-hsp65 assay. From all goats, 60% (n = 36/60), 3.3% (n = 2/60), and 36.7% (n = 22/60) showed positive, inconclusive, and negative reactions, respectively. Out of 36 goats with ICCTT positive, 75% (n = 27/36) had isolation of mycobacteria and were detected M. bovis by gyrB-RFLP. Molecular diagnosis and histopathological findings compatible with tuberculosis showed 86.1% (n = 31/36) concordance with the ICCTT. When compared ICCTT with M. bovis isolation, gyrB-RFLP, and histopathology, the better arithmetic means of sensitivity and specificity were 2.5 mm for ICCTT compared with M. bovis isolation and gyrB-RFLP, and 4.55 mm when compared with histopathology. Both receiver operating characteristic (ROC) curves presented statistical significance (P < 0.001). The identification of other mycobacteria, e.g., M. kansasii, M. flavescens, M. avium, M. florentinum, M. lentiflavum, M. simiae, and Corynebacterium pseudotuberculosis, not influenced positive results in ICCTT. The concordance between bacteriological, histopathological, and molecular identification with ICCTT findings indicate that the tuberculin test may be used as a valuable tool for diagnosis of caprine tuberculosis and reinforce the importance of association of methods to diagnostic of the disease from animal origin. (AU)

• Processo FAPESP: 06/58323-6 - Avaliacao do teste cervical comparativo no diagnostico imunoalergico da tuberculose em caprinos (capra hircus).
• Beneficiário: Carlos Augusto Scacchetti de Almeida
• Modalidade de apoio: Bolsas no Brasil - Mestrado