oorly Conserved P15 Proteins of Cileviruses Retain Elements of Common Ancestry and Putative Functionality: A Theoretical Assessment on the Evolution of Cilevirus Genome

Autor(es)

Ramos-Gonzalez, Pedro L. ^[1] ; Pons, Tirso ^[2] ; Chabi-Jesus, Camila ^{[3, 1]} ; Arena, Gabriella Dias ^[1] ; Freitas-Astua, Juliana ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Inst Biol Sao Paulo, Lab Biol Mol Aplicada, Sao Paulo - Brazil

^[2] Natl Ctr Biotechnol CNB CSIC, Madrid - Spain

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Piracicaba - Brazil

^[4] Embrapa Mandioca & Fruticultura, Cruz Das Almas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 12, NOV 5 2021.
• Citações Web of Science: 0

Resumo

The genus Cilevirus groups enveloped single-stranded (+) RNA virus members of the family Kitaviridae, order Martellivirales. Proteins P15, scarcely conserved polypeptides encoded by cileviruses, have no apparent homologs in public databases. Accordingly, the open reading frames (ORFs) p15, located at the 5 `-end of the viral RNA2 molecules, are considered orphan genes (ORFans). In this study, we have delved into ORFs p15 and the relatively poorly understood biochemical properties of the proteins P15 to posit their importance for viruses across the genus and theorize on their origin. We detected that the ORFs p15 are under purifying selection and that, in some viral strains, the use of synonymous codons is biased, which might be a sign of adaptation to their plant hosts. Despite the high amino acid sequence divergence, proteins P15 show the conserved motif {[}FY]-L-x(3)-{[}FL]-H-x-x-{[}LIV]-S-C-x-C-x(2)-C-x-G-x-C, which occurs exclusively in members of this protein family. Proteins P15 also show a common predicted 3D structure that resembles the helical scaffold of the protein ORF49 encoded by radinoviruses and the phosphoprotein C-terminal domain of mononegavirids. Based on the 3D structural similarities of P15, we suggest elements of common ancestry, conserved functionality, and relevant amino acid residues. We conclude by postulating a plausible evolutionary trajectory of ORFans p15 and the 5 `-end of the RNA2 of cileviruses considering both protein fold superpositions and comparative genomic analyses with the closest kitaviruses, negeviruses, nege/kita-like viruses, and unrelated viruses that share the ecological niches of cileviruses. (AU)

• Processo FAPESP: 19/02137-0 - P61 dos cilevirus: caracterização bioquímica e funcional
• Beneficiário: Gabriella Dias Arena
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/25078-9 - Genômica e transcriptômica das relações vírus-vetor-hospedeira nos patossistemas dos vírus transmitidos por Brevipalpus; sistemática e evolução de Brevipalpus e seus endosimbiontes; novas estratégias no manejo da Leprose do citros no Estado de São Paulo
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/50222-0 - Compreensão sobre a biologia molecular e ecologia da relações planta-vírus-vetor: rumo a estratégias sustentáveis e integradas de manejo de vírus
• Beneficiário: Juliana de Freitas Astúa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/15413-2 - Glicoproteínas putativas de vírus transmitidos por ácaros Brevipalpus e seu papel na formação das estruturas virais
• Beneficiário: Camila Chabi de Jesus
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado