Resistance exercise shifts the balance of renin-angiotensin system toward ACE2/Ang 1-7 axis and reduces inflammation in the kidney of diabetic rats

Autor(es)

Santos, Ralmony de Alcantara ^[1] ; Guzzoni, Vinicius ^[2] ; Silva, Kleiton Augusto Santos ^{[1, 3]} ; Aragao, Danielle Sanches ^[1] ; Vieira, Rodolfo de Paula ^[2] ; Bertoncello, Nadia ^[2] ; Schor, Nestor ^[1] ; Aimbire, Flavio ^[2] ; Casarini, Dulce Elena ^[1] ; Cunha, Tatiana Sousa ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Med, Nephrol Div, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Inst Sci & Technol, Sao Jose Dos Campos - Brazil

^[3] Univ Missouri, Dept Biomed Sci, Columbia, MO - USA

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 287, DEC 15 2021.
• Citações Web of Science: 0

Resumo

Aims: We aimed to determine whether resistance training (RT) regulates renal renin-angiotensin system (RAS) components and inflammatory mediators in diabetic rats. Main methods: Male Wistar rats (3 months old) were randomly assigned into four groups: non-trained (NT), trained (T), non-trained + diabetes (NTD) and trained +diabetes (TD). Diabetes was induced by streptozotocin (50 mg/kg, Sigma Chemical Co., St. Louis, MO, USA), before RT protocol. Trained rats performed RT protocol on a 110-cm ladder (8 ladder climbs, once/day, 5 days/week, 8 weeks), carrying a load corresponding to 50-80% of maximum carrying capacity. Blood glucose, albuminuria and urinary volume were measured. Renal levels of angiotensin peptides (angiotensin I, II and 1-7), inflammatory markers, and also the activities of angiotensinconverting enzyme (ACE) and ACE2 were determined. Key findings: Blood glucose and urinary volume were elevated in diabetic animals, and RT decreased albuminuria, renal Ang I and Ang II levels in diabetic rats. RT shifted the balance of renal RAS toward ACE2/Ang 1-7 axis in TD group, and mitigated the high levels of interleukin (IL)-10, IL-1 beta and cytokine-induced neutrophil chemoattractant 1 (CINC) in the context of diabetes. Strong positive correlations were found between albuminuria and Ang II, IL-10 and IL-1 beta. On the other hand, intrarenal Ang 1-7 levels were negatively correlated with IL-10 and IL-1 beta levels. Significance: RT improved kidney function by modulating intrarenal RAS toward ACE2/Ang 1-7 axis and inflammatory cytokines. RT represents a reasonable strategy to improve the renal complications induced by diabetes, counteracting nephropathy-associated maladaptive responses. (AU)

• Processo FAPESP: 13/20034-7 - Avaliação dos efeitos do treinamento físico resistido e do aeróbio sobre a cardiomiopatia diabética: participação das vias de sinalização do metabolismo energético e do sistema renina angiotensina
• Beneficiário: Ralmony de Alcantara Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado