ranscriptomic and histological analysis of exposed facial skin areas wrinkled or not and unexposed ski

Autor(es):Mostrar menos -

Martin, Renan Paulo ^[1] ; Varela, Patricia ^[2] ; Gomes, Caio Peres ^[2] ; Marins, Maryana Mara ^[2] ; Filippelli-Silva, Rafael ^[2] ; Yarak, Samira ^[3] ; Soares, Juliana L. M. ^[3] ; Sanudo, Adriana ^[4] ; Idkowiak-Baldys, Jolanta ^[5] ; Chen, Siming ^[5] ; Hwang, Cheng ^[5] ; Zhuang, Yong ^[5] ; Lyga, John ^[5] ; Pesquero, Joao Bosco ^[2] ; Bagatin, Edileia ^[3]

Afiliação do(s) autor(es)

^[1] Johns Hopkins Sch Med, McKusick Nathans Dept Genet Med, Baltimore, MD 21205 - USA

^[2] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Dermatol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Prevent Med, Sao Paulo - Brazil

^[5] Avon Prod Inc, New York, NY 10901 - USA

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 49, n. 3 DEC 2021.
• Citações Web of Science: 0

Resumo

Background Skin aging involves genetic, environmental and hormonal factors. Facial wrinkles also depend on muscular activity. Gene expression investigation may be useful for new anti-aging products. Methods and Results To evaluate structure and gene expression differences among exposed and unexposed skin in menopausal women. Cross-sectional study, including 15 menopausal women, 55-65 years, phototype III; photo-exposed, periorbital wrinkles (A1), preauricular, not wrinkled (A2), and unexposed gluteal (A3) areas were described and compared by non-invasive measures, histology, immunohistochemistry and gene expression (RNASeq); participants mean age was 61yo, presenting moderate periorbital wrinkles and light facial photodamage. Higher roughness, wrinkles number and echogenicity were observed in A1 and A2 versus A3. Decreased epidermal thickness and dermal collagen IV were demonstrated in A1 versus A2 and A3. Exposed areas impacted different pathways compared to unexposed. Exposed wrinkled skin (A1) showed impact on cell movement with decreased inflammatory activation state. Pathways related to lipid and aminoacids metabolism were modulated in non-wrinkled exposed (A2) compared to unexposed (A3) skin. Conclusions Expected histological findings and gene expression differences among areas were observed. Photoaging in menopausal women may modulate lipid and aminoacids metabolism and decrease inflammatory and keratinization pathways, cellular homeostasis, immune response, fibrogenesis and filament formation. These findings may help development of new therapies for skin health and aging control. (AU)

• Processo FAPESP: 14/27198-8 - Estabelecimento de um centro de pesquisa genética e molecular para desafios clínicos
• Beneficiário: João Bosco Pesquero
• Modalidade de apoio: Auxílio à Pesquisa - Temático