The Use of Menthol in Skin Wound Healing-Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization

Autor(es)

Rozza, Ariane Leite ^[1] ; Beserra, Fernando Pereira ^[1] ; Vieira, Ana Julia ^[1] ; Oliveira de Souza, Eduardo ^[1] ; Hussni, Carlos Alberto ^[2] ; Martinez, Emanuel Ricardo Monteiro ^[1] ; Nobrega, Rafael Henrique ^[1] ; Pellizzon, Claudia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Struct & Funct Biol, Dr Antonio Celso Zanin St 250, BR-18618689 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Surg & Vet Anesthesiol, Dr Walter M Correa St, BR-18618689 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 13, n. 11 NOV 2021.
• Citações Web of Science: 0

Resumo

Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-alpha and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization. (AU)

• Processo FAPESP: 13/23340-1 - Processo cicatricial em pele sob tratamento com monoterpeno (mentol) e triterpeno (lupeol) em modelos experimentais
• Beneficiário: Claudia Helena Pellizzon
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/22202-4 - Uso de creme à base de mentol na cicatrização de feridas cutâneas
• Beneficiário: Ariane Leite Rozza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/06636-0 - Avaliação antioxidante e anti-inflamatória do mentol no processo cicatricial de pele
• Beneficiário: Eduardo Oliveira de Souza
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/13307-5 - Mecanismos fisiológicos envolvidos na cicatrização cutânea após tratamento com mentol em ratos diabéticos e não-diabéticos
• Beneficiário: Ariane Leite Rozza
• Modalidade de apoio: Auxílio à Pesquisa - Regular