Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals

Autor(es)

Leroy, Kaat ^[1] ; Pieters, Alanah ^[1] ; Cooreman, Axelle ^[1] ; Van Campenhout, Raf ^[1] ; Cogliati, Bruno ^[2] ; Vinken, Mathieu ^[1]

Afiliação do(s) autor(es)

^[1] Vrije Univ Brussel, Dept Pharmaceut & Pharmacol Sci, Ent Vitro Toxicol & Dermatocosmetol, Laarbeeklaan 103, B-1090 Brussels - Belgium

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 21 NOV 2021.
• Citações Web of Science: 0

Resumo

Connexin-based channels play key roles in cellular communication and can be affected by deleterious chemicals. In this study, the effects of various genotoxic carcinogenic compounds, non-genotoxic carcinogenic compounds and non-carcinogenic compounds on the expression and functionality of connexin-based channels, both gap junctions and connexin hemichannels, were investigated in human hepatoma HepaRG cell cultures. Expression of connexin26, connexin32, and connexin43 was evaluated by means of real-time reverse transcription quantitative polymerase chain reaction analysis, immunoblot analysis and in situ immunostaining. Gap junction functionality was assessed via a scrape loading/dye transfer assay. Opening of connexin hemichannels was monitored by measuring extracellular release of adenosine triphosphate. It was found that both genotoxic and non-genotoxic carcinogenic compounds negatively affect connexin32 expression. However, no specific effects related to chemical type were observed at gap junction or connexin hemichannel functionality level. (AU)

• Processo FAPESP: 18/10953-9 - As conexinas, panexinas e seus hemi(canais) são novos biomarcadores e alvos terapêuticos no prognóstico e terapia do câncer hepático?
• Beneficiário: Bruno Cogliati
• Modalidade de apoio: Auxílio à Pesquisa - Regular