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Enzymatic Degumming of Rice Bran Oil Using Different Commercial Phospholipases and Their Cocktails

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Autor(es):
Rodrigues, Mayara S. [1] ; Dos Passos, Rafaela M. [1] ; Pontes, Paula V. de A. [1] ; Ferreira, Marcela C. [2] ; Meirelles, Antonio J. A. [1] ; Stevens, Christian V. [3] ; Maximo, Guilherme J. [1] ; Sampaio, Klicia A. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Engn, BR-13083862 Campinas - Brazil
[2] Univ Estadual Campinas, Fac Technol, BR-13484332 Limeira - Brazil
[3] Univ Ghent, Fac Biosci Engn, Dept Green Chem & Technol, B-9000 Ghent - Belgium
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: LIFE-BASEL; v. 11, n. 11 NOV 2021.
Citações Web of Science: 0
Resumo

Rice bran oil is a highly nutritious vegetable oil, as it is rich in tocols and gamma-oryzanol. Degumming is the first step in the vegetable oil refining process, and its main objective is the removal of phospholipids or gums. In the present study, enzymatic degumming trials were performed on crude rice bran oil using the phospholipases PLA1, Purifine(R) PLC, their mixture (PLA1/PLC), and a cocktail known as Purifine(R) 3G. Enzymatic degumming applying 50 mg/kg of PLA1 for 120 min resulted in a residual phosphorus content of 10.4 mg/kg and an absolute free fatty acid increase of 0.30%. Enzymatic degumming applying 300 mg/kg of Purifine(R) PLC for 120 min at 60 \& DEG;C resulted in a residual phosphorus content of 67 mg/kg and an absolute diacylglycerol increase of 0.41%. The mixture of phospholipases and the cocktail presented approximately 5 mg/kg of residual phosphorus content after the reaction times. For all degumming processes, the preservation of minor components such as tocols and gamma-oryzanol were observed. These results indicate that the use of enzyme mixtures or their cocktails to attain low phosphorus content and high diacylglycerol/free fatty acid conversion during enzymatic degumming is a viable alternative. (AU)

Processo FAPESP: 14/21252-0 - Equilíbrio e processos de produção de biocombustíveis e bioprodutos
Beneficiário:Antonio José de Almeida Meirelles
Modalidade de apoio: Auxílio à Pesquisa - Temático